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Cryo-electron microscopy of viruses


Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional electron microscopy. Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare favourably with any other electron microscopical method.

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Adrian, M., Dubochet, J., Lepault, J. et al. Cryo-electron microscopy of viruses. Nature 308, 32–36 (1984).

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