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Expression of functional acetylcholine receptor from cloned cDNAs

Nature volume 307, pages 604–608 (1984)Cite this article

Abstract

The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin binding activity.

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References

  1. Karlin, A. in Cell Surface Reviews Vol. 6 (eds Cotman, C. W., Poste, G. & Nicolson, G. L.) 191–260 (North-Holland, Amsterdam, 1980).

    Google Scholar 

  2. Changeux, J.-P. Harvey Lect. 75, 85–254 (1981).

    CAS  Google Scholar 

  3. Conti-Tronconi, B. M. & Raftery, M. A. A. Rev. Biochem. 51, 491–530 (1982).

    Article  CAS  Google Scholar 

  4. Noda, M. et al. Nature 299, 793–797 (1982).

    Article  ADS  CAS  Google Scholar 

  5. Noda, M. et al. Nature 301, 251–255 (1983).

    Article  ADS  CAS  Google Scholar 

  6. Noda, M. et al. Nature 302, 528–532 (1983).

    Article  ADS  CAS  Google Scholar 

  7. Noda, M. et al. Nature 305, 818–823 (1983).

    Article  ADS  CAS  Google Scholar 

  8. Claudio, T., Ballivet, M., Patrick, J. & Heinemann, S. Proc. natn. Acad. Sci. U.S.A. 80, 1111–1115 (1983).

    Article  ADS  CAS  Google Scholar 

  9. Sumikawa, K. et al. Nucleic Acids Res. 10, 5809–5822 (1982).

    Article  CAS  Google Scholar 

  10. Devillers-Thiery, A., Giraudat, J., Bentaboulet, M. & Changeux, J.-P. Proc. natn. Acad. Sci. U.S.A. 80, 2067–2071 (1983).

    Article  ADS  CAS  Google Scholar 

  11. Lindstrom, J. & Dau, P. A. Rev. Pharmac. Tox. 20, 337–362 (1980).

    Article  CAS  Google Scholar 

  12. O'Hare, K., Benoist, C. & Breathnach, R. Proc. natn. Acad. Sci. U.S.A. 78, 1527–1531 (1981).

    Article  ADS  CAS  Google Scholar 

  13. Okayama, H. & Berg, P. Molec. cell. Biol. 2, 161–170 (1982).

    Article  CAS  Google Scholar 

  14. Gluzman, Y. Cell 23, 175–182 (1981).

    Article  CAS  Google Scholar 

  15. Graham, F. L. & van der Eb, A. J. Virology 52, 456–467 (1973).

    Article  CAS  Google Scholar 

  16. Berk, A. J. & Sharp, P. A. Cell 12, 721–732 (1977).

    Article  CAS  Google Scholar 

  17. Haegeman, G. & Fiers, W. J. Virol. 35, 955–961 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Benoist, C. & Chambon, P. Nature 290, 304–310 (1981).

    Article  ADS  CAS  Google Scholar 

  19. Gruss, P. & Khoury, G. Proc. natn. Acad. Sci. U.S.A. 78, 133–137 (1981).

    Article  ADS  CAS  Google Scholar 

  20. Gurdon, J. B. The Control of Gene Expression in Animal Development (Clarendon, Oxford, 1974).

    Google Scholar 

  21. Sumikawa, K., Houghton, M. Em tage, J. S., Richards, B. M. & Barnard, E. A. Nature 292, 862–864 (1981).

    Article  ADS  CAS  Google Scholar 

  22. Barnard, E. A., Miledi, R. & Sumikawa, K. Proc. R. Soc. 215, 241–246 (1982).

    ADS  CAS  Google Scholar 

  23. Kusano, K., Miledi, R. & Stinnakre, J. Nature 270, 739–741 (1977).

    Article  ADS  CAS  Google Scholar 

  24. Kusano, K., Miledi, R. & Stinnakre, J. J. Physiol., Lond. 328, 143–170 (1982).

    Article  CAS  Google Scholar 

  25. Gullick, W. J. & Lindstrom, J. M. Biochemistry 22, 3312–3320 (1983).

    Article  CAS  Google Scholar 

  26. Haggerty, J. G. & Froehner, S. C. J. biol. Chem. 256, 8294–8297 (1981).

    CAS  PubMed  Google Scholar 

  27. Tzartos, S. J. & Changeux, J.-P. EMBO J. 2, 381–387 (1983).

    Article  CAS  Google Scholar 

  28. Weber, M. & Changeux, J.-P. Molec. Pharmac. 10, 15–34 (1974).

    CAS  Google Scholar 

  29. Miledi, R. J. Physiol., Lond. 151, 1–23 (1960).

    Article  CAS  Google Scholar 

  30. Weinberg, C. B. & Hall, Z. W. Proc. natn. Acad. Sci. U.S.A. 76, 504–508 (1979).

    Article  ADS  CAS  Google Scholar 

  31. Soberon, X., Covarrubias, L. & Bolivar, F. Gene 9, 287–305 (1980).

    Article  CAS  Google Scholar 

  32. Mulligan, R. C. & Berg, P. Science 209, 1422–1427 (1980).

    Article  ADS  CAS  Google Scholar 

  33. Mishina, M. et al. EMBO J. l, 1533–1538 (1982).

    Article  Google Scholar 

  34. Nakamura, M., Nakanishi, S., Sueoka, S., Imura, H. & Numa, S. Eur. J. Biochem. 86, 61–66 (1978).

    Article  CAS  Google Scholar 

  35. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  36. Nakanishi, S. et al. Proc. natn. Acad. Sci. U.S.A. 73, 4319–4323 (1976).

    Article  ADS  CAS  Google Scholar 

  37. King, J. & Laemmli, U. K. J. molec. Biol. 62, 465–477 (1971).

    Article  CAS  Google Scholar 

  38. Chamberlain, J. P. Analyt. Biochem. 98, 132–135 (1979).

    Article  ADS  CAS  Google Scholar 

  39. Kaneda, N., Tanaka, F., Kohno, M., Hayashi, K. & Yagi, K. Archs Biochem. Biophys. 218, 376–383 (1982).

    Article  CAS  Google Scholar 

  40. Weber, M. & Changeux, J.-P. Molec. Pharmac. 10, 1–14 (1974).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Medical Chemistry, Kyoto University Faculty of Medicine, Yoshida, Kyoto, 606, Japan

    Masayoshi Mishina, Tomohiro Kurosaki, Takamasa Tobimatsu, Yuuki Morimoto, Masaharu Noda, Tokuo Yamamoto, Mineko Terao & Shosaku Numa

  2. Department of Physiology, Kyoto University Faculty of Medicine, Yoshida, Kyoto, 606, Japan

    Tomoyuki Takahashi & Motoy Kuno

  3. The Salk Institute for Biological Studies, San Diego, California, 92138, USA

    Jon Lindstrom

Authors
  1. Masayoshi Mishina
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  2. Tomohiro Kurosaki
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  5. Masaharu Noda
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Mishina, M., Kurosaki, T., Tobimatsu, T. et al. Expression of functional acetylcholine receptor from cloned cDNAs. Nature 307, 604–608 (1984). https://doi.org/10.1038/307604a0

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  • DOI: https://doi.org/10.1038/307604a0

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