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Acute stress facilitates long-lasting changes in cholinergic gene expression

A Corrigendum to this article was published on 25 November 2015

Abstract

Acute traumatic stress may lead to post-traumatic stress disorder (PTSD)1, which is characterized by delayed neuropsychiatric symptoms including depression, irritability, and impaired cognitive performance2. Curiously, inhibitors of the acetylcholine-hydrolysing enzyme acetylcholinesterase may induce psychopathologies that are reminiscent of PTSD3,4. It is unknown how a single stressful event mediates long-term neuronal plasticity. Moreover, no mechanism has been proposed to explain the convergent neuropsychological outcomes of stress and of acetylcholinesterase inhibition. However, acute stress elicits a transient increase in the amounts released of the neurotransmitter acetylcholine and a phase of enhanced neuronal excitability5. Inhibitors of acetylcholinesterase also promote enhanced electrical brain activity6, presumably by increasing the survival of acetylcholine at the synapse. Here we report that there is similar bidirectional modulation of genes that regulate acetylcholine availability after stress and blockade of acetylcholinesterase. These calcium-dependent changes in gene expression coincide with phases of rapid enhancement and delayed depression of neuronal excitability. Both of these phases are mediated by muscarinic acetylcholine receptors. Our results suggest a model in which robust cholinergic stimulation triggers rapid induction of the gene encoding the transcription factor c-Fos. This protein then mediates selective regulatory effects on the long-lasting activities of genes involved in acetylcholine metabolism.

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Figure 1: Acute stress and anticholinesterases modulate CNS gene expression similarly.
Figure 2: Delayed suppression of the hyperexcitation evoked by AChE inhibition.
Figure 3: Physiological and transcriptional responses both depend on intracellular Ca2+ accumulation and Na+ influx.
Figure 4: Long-term changes in AChE activity following stress.
Figure 5: Selective induction of read-through AChE mRNA following stress and AChE inhibition.

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Acknowledgements

We thank M. J. Gutnick for inspiration and discussion, and M. Sternfeld for recombinant cholinesterase variants. This research was supported by grants from the US Army Medical Research and Development Command, the Israel Science Fund, the USA–Israel Binational Science Foundation, the Israeli Health Ministry and Ester/Medica Neuroscience (to H.S.). A.F. was a post-doctoral fellow of the Smith Psychobiology Fund and an incumbent of the Teva research prize for young investigators.

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Correspondence to Hermona Soreq.

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Kaufer, D., Friedman, A., Seidman, S. et al. Acute stress facilitates long-lasting changes in cholinergic gene expression. Nature 393, 373–377 (1998). https://doi.org/10.1038/30741

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