Abstract
CBA/N mice carrying the X-linked immune deficiency gene (xid) have fewer splenic B cells than normal CBA mice and are unresponsive to a certain class of antigens1. Studies of B-cell surface-marker expression2 and immune responsiveness3 have led to the commonly accepted idea that the B cells in adult xid mice are immature and resemble the B cells of young (1–3 week old) normal mice. That is, like young animals, xid mice lack cells in the most numerous of three IgM/IgD B-cell sub-populations (designated I in Fig. 1a, b) present in adult spleen4,5. We now report, however, that this picture is an oversimplification and that in fact the B cells in adult xid mice differ from those present in either adult or young normal mice. Using quantitative three-colour fluorescence-activated cell sorter (FACS) analyses, we have compared the correlated expression of IgM, IgD and a newly discovered B-lymphocyte antigen (BLA-1) on splenic B cells in normal and xid mice. We show here (1) that most B cells in adult xid mice (as in normals) are BLA-1− whereas all B cells in young animals are BLA-1+; (2) that the major difference in the IgM/IgD B-cell subpopulations found between xid and normal mice is limited to the BLA-1− cells; and (3) that xid mice have increased numbers of BLA-1+ population III B cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Scher, I., Frantz, M. M. & Steinberg, A. D. J. Immun. 110, 1396–1401 (1973).
Scher, I., Sharrow, S. O. & Paul, W. E. J. exp. Med. 144, 507–518 (1977).
Mosier, D. E. et al. Immun. Rev. 37, 89–104 (1977).
Hardy, R. R., Hayakawa, K., Haaijman, J. & Herzenberg, L. A. Nature 297, 589–591 (1982).
Hardy, R. R., Hayakawa, K., Haaijman, J. & Herzenberg, L. A. in Immunoglobulin D: Structure and Function (eds Thorbecke, G. J. & Leslie, G. A.) 112–121 (New York Academy of Science, 1982).
Ledbetter, J. A. & Herzenberg, L. A. Immun. Rev. 47, 63–90 (1979).
Huber, B., Gershon, R. K. & Cantor, H. J. exp. Med. 145, 10–20 (1977).
Ahmed, A. et al. J. exp. Med. 145, 101–110 (1977).
Subbarao, B. et al. J. Immun. 122, 2279–2285 (1979).
Oi, V. T., Glazer, A. N. & Stryer, L. J. Cell Biol. 93, 981–986 (1982).
Loken, M. R., Parks, D. R. & Herzenberg, L. A. J. Histochem. Cytochem. 25, 899–907 (1977).
Parks, D. R., Hardy, R. R. & Herzenberg, L. A. Immun. Today 4(5), 145–150 (1983).
Herzenberg, L. A. et al. Immun. Rev. 67, 5–31 (1982).
Hayakawa, K., Hardy, R. R., Parks, D. R. & Herzenberg, L. A. J. exp. Med. 157, 202–218 (1983).
Metcalf, E. S. & Klinman, N. R. J. exp. Med. 143, 1327–1340 (1976).
Teal, J. M. & Klinman, N. R. Nature 288, 385–387 (1980).
Nossal, G. J. V. & Pike, B. L. in Immunology 80—Progress in Immunology IV (eds Fougereau, M. & Dausset, J.) 136–152 (Academic, London, 1980).
Singer, A. et al. Immun. Rev. 64, 137–160 (1982).
Kincade, P. W., Lee, G., Sun, L. & Watanabe, T. J. immun. Meth. 42, 17–26 (1981).
Oi, V. T., Jones, P. P., Goding, J. W., Herzenberg, L. A. & Herzenberg, L. A. Curr. Topics Microbiol. Immun. 8, 115–129 (1978).
Carlsson, J., Drevin, H. & Axen, R. Biochem. J. 173, 723–737 (1978).
Lee, A. C. J., Powell, J. E., Tregear, G. W., Niall, H. D. & Stevens, V. C. Molec. Immun. 17, 749–756 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hardy, R., Hayakawa, K., Parks, D. et al. Demonstration of B-cell maturation in X-linked immunodeficient mice by simultaneous three-colour immunofluorescence. Nature 306, 270–272 (1983). https://doi.org/10.1038/306270a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/306270a0
This article is cited by
-
Mediation of transitional B cell maturation in the absence of functional Bruton’s tyrosine kinase
Scientific Reports (2017)
-
Modulating proximal cell signaling by targeting Btk ameliorates humoral autoimmunity and end-organ disease in murine lupus
Arthritis Research & Therapy (2012)
-
The follicular versus marginal zone B lymphocyte cell fate decision
Nature Reviews Immunology (2009)
-
A probabilistic view of immunology: Drawing parallels with physics
Immunology & Cell Biology (2007)
-
Antibody regulation of B cell development
Nature Immunology (2000)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.