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Evolutionary adaptation of plasmid-encoded enzymes for degrading nylon oligomers

Abstract

Flavobacterium sp. KI72 metabolizes 6-aminohexanoic acid cyclic dimer, a by-product of nylon manufacture1, through two newly evolved enzymes, 6-aminohexanoic acid cyclic dimer hydrolase (EI)2 and 6-aminohexanoic acid linear oligomer hydrolase (EII)3. These enzymes are active towards man-made compounds, the cyclic dimer and linear oligomers of 6-aminohexanoic acid respectively, but not towards any of the natural amide bonds tested2,3. The structural genes of EI (nylA) and EII (nylB) are encoded on pOAD2, one of three plasmids harboured in Flavobacterium sp. KI724,5. This plasmid contains two kinds of repeated sequence (RS-I and RS-II); one of the two RS-II sequences, RS-IIA, contains the nylB gene6, while the other, RS-IIB, contains a homologous nylB′ gene. From comparisons of the nucleotide sequences and gene products of the nylB and nylB′ genes, we now conclude that EII enzyme is newly evolved by gene duplication followed by base substitutions on the same plasmid.

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References

  1. 1

    Kinoshita, S., Kageyama, S., Iba, K., Yamada, Y. & Okada, H. Agric. biol. Chem. 39, 1219–1223 (1975).

    CAS  Google Scholar 

  2. 2

    Kinoshita, S. et al. Eur. J. Biochem. 80, 489–495 (1977).

    CAS  Article  Google Scholar 

  3. 3

    Kinoshita, S. et al. Eur. J. Biochem. 116, 547–551 (1981).

    CAS  Article  Google Scholar 

  4. 4

    Negoro, S. et al. J. Bact. 143, 238–245 (1980).

    CAS  PubMed  Google Scholar 

  5. 5

    Negoro, S. & Okada, H. Agric. biol. Chem. 46, 745–750 (1982)

    CAS  Google Scholar 

  6. 6

    Negoro, S., Taniguchi, T., Kanaoka, M., Kimura, H. & Okada, H. J. Bact. 155, 22–31 (1983).

    CAS  PubMed  Google Scholar 

  7. 7

    Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    CAS  Article  Google Scholar 

  8. 8

    Bolivar, F. et al. Gene 2, 95–113 (1977).

    CAS  Article  Google Scholar 

  9. 9

    Backman, K., Ptashne, M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 73, 4174–4178 (1976).

    ADS  CAS  Article  Google Scholar 

  10. 10

    Shine, J. & Dalgarno, L. Proc. natn. Acad. Sci. U.S.A. 71, 1342–1346 (1974).

    ADS  CAS  Article  Google Scholar 

  11. 11

    Gold, L. et al. A. Rev. Microbiol. 35, 365–403 (1981).

    CAS  Article  Google Scholar 

  12. 12

    Jacobsen, H., Klenow, H. & Overgaard-Hansen, K. Eur. J. Biochem. 45, 623–627 (1974).

    CAS  Article  Google Scholar 

  13. 13

    Ouchterlony, Ö Acta path. microbiol. 26, 507–515 (1949).

    CAS  Article  Google Scholar 

  14. 14

    Paterson, A. & Clarke, P. H. J. gen. Microbiol. 114, 75–85 (1979).

    CAS  Article  Google Scholar 

  15. 15

    Smith, H. O. & Birnstiel, M. L. Nucleic Acids Res. 3, 2387–2398 (1976).

    CAS  Article  Google Scholar 

  16. 16

    Tu, C. D. & Cohen, S. N. Gene 10, 177–183 (1980).

    CAS  Article  Google Scholar 

  17. 17

    Laurell, C. B. Analyt. Biochem. 15, 45–52 (1966).

    CAS  Article  Google Scholar 

Download references

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Okada, H., Negoro, S., Kimura, H. et al. Evolutionary adaptation of plasmid-encoded enzymes for degrading nylon oligomers. Nature 306, 203–206 (1983). https://doi.org/10.1038/306203a0

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