The haematopoietic system is made up of a hierarchy of cells with different developmental, functional and proliferative capacities. Although cellular diversity appears to arise from the commitment and maturation of stem cells, the molecular basis for this differentiation process is unknown. The introduction of cloned DNA sequences into haematopoietic progenitor cells would provide a novel approach for studying this differentiating in vivo system. One laboratory has reported DNA-mediated transfer of genes into mouse bone marrow cells1,2. However, retroviruses offer a number of advantages over DNA-mediated gene transfer procedures, including high efficiency infection of a wide range of cell types in vitro and in vivo, stable and low copy integration into the host chromosome, and a defined integrated provirus structure. For these reasons recombinant DNA techniques have been utilized to construct high efficiency retrovirus vectors expressing foreign genes3–8. We demonstrate here, using such a retrovirus vector, the transfer of a dominant selectable drug-resistance gene into defined classes of mouse haematopoietic progenitor cells. These observations should facilitate the development of molecular genetic approaches to fundamental and clinical problems in haematopoiesis.
Subscribe to Journal
Get full journal access for 1 year
only $3.90 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Tax calculation will be finalised during checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Cline, M. J. et al. Nature 284, 422–425 (1980).
Mercola, K. E., Stang, H. D., Browne, J., Salser, W. & Cline, M. J. Science 208, 1033–1035 (1980).
Shimotohno, K. & Temin, H. Cell 26, 67–78 (1981).
Wei, C.-M., Gibson, M., Spear, P. G. & Scolnick, E. M. J. Virol. 39, 935–944 (1981).
Tabin, C. J., Hoffman, J. W., Goff, S. P. & Weinberg, R. A. Molec. cell. Biol. 2, 426–436 (1982).
Sorge, J. & Hughes, J. H. J. Molec. appl. Genet. 1, 547–559 (1982).
Joyner, A. L. & Bernstein, A. in Gene Transfer and Cancer (eds Pearson, M. & Sternberg, W.) (Raven, New York, in the press).
Joyner, A. & Bernstein, A. Molec. cell. Biol. (in the press).
Colbere-Garapin, F., Horodniceanu, F., Kourilsky, P. & Garapin, A.-C. J. molec. Biol. 150, 1–14 (1981).
Southern, P. J. & Berg, P. Molec. appl. Genet. 1, 327–341 (1982).
Johnson, G. R. & Metcalf, D. Proc. natn. Acad. Sci. U.S.A. 74, 3879–3882 (1977).
Manzari, V. et al. Proc. natn. Acad. Sci. U.S.A. 80, 11–15 (1983).
Mann, R., Mulligan, R. C. & Baltimore, D. Cell 33, 153–159 (1983).
Wahl, G., Stern, M. & Stark, G. Proc. natn. Acad. Sci. U.S.A. 76, 3683–3687 (1979).
About this article
Cite this article
Joyner, A., Keller, G., Phillips, R. et al. Retrovirus transfer of a bacterial gene into mouse haematopoietic progenitor cells. Nature 305, 556–558 (1983). https://doi.org/10.1038/305556a0
Identification of immature podocyte specific antigen using retrovirus-mediated gene transfer and cell sorting
Clinical and Experimental Nephrology (2005)
Retrovirus-mediated gene transfer into CD4+ and CD8+ human T cell subsets derived from tumor-infiltrating lymphocytes and peripheral blood mononuclear cells
Cancer Immunology Immunotherapy (1991)
Gonadotropes in a novel rat pituitary tumor cell line, RC-4B/C. Establishment and partial characterization of the cell line
In Vitro Cellular & Developmental Biology (1990)