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Expression of I–Ak class II genes in mouse L cells after DNA-mediated gene transfer


The I–region of the mouse H-2 complex encodes I-region-associated or Ia antigens which are involved in lymphocyte interactions and in the regulation of immune responsiveness. Two Ia antigens, termed I–A and I–E, have been identified; both are cell-surface integral membrane glycoproteins which consist of two noncovalently linked polypeptide chains designated α (molecular weight (MW) 34,000) and β ( 28,000 MW)1. Their expression is restricted to B lymphocytes and to antigen-presenting cells such as macrophages. Inside the cell, these αβ dimers are associated noncovalently with a nonpolymorphic chain of 31,000 MW designated the invariant (Ii) chain2. Cloning of 200 kilobases (kb) of the I-region from mice of the H–2d and H–2k haplotypes was accomplished recently in our laboratory3,4, and the genes encoding the four polypeptide chains, Aα, Aβ, Eα and Eβ were identified. We now describe the successful expression of I–Ak molecules in transfected mouse L cells.

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Malissen, B., Steinmetz, M., McMillan, M. et al. Expression of I–Ak class II genes in mouse L cells after DNA-mediated gene transfer. Nature 305, 440–443 (1983).

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