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Quantification of Ca2+-activated K+ channels under hormonal control in pig pancreas acinar cells


Ca2+- and voltage-activated K+ channels are found in many electrically excitable cells and have an important role in regulating electrical activity1–4. Recently, the large K+ channel has been found in the baso-lateral plasma membranes of salivary gland acinar cells, where it may be important in the regulation of salt transport5. Using patch-clamp methods6,7 to record single-channel currents from excised fragments of baso-lateral acinar cell membranes in combination with current recordings from isolated single acinar cells and two- and three-cell clusters, we have now for the first time characterized the K+ channels quantitatively. In pig pancreatic acini there are 25–60 K+ channels per cell with a maximal single channel conductance of about 200 pS. We have quantified the relationship between internal ionized Ca2+ concentration ([Ca2+]i) membrane potential and open-state probability (p) of the K+ channel. By comparing curves obtained from excised patches relating membrane potential to p, at different levels of [Ca2+]i, with similar curves obtained from intact cells, [Ca2+]i in resting acinar cells was found to be between 10−8 and 10−7 M. In microelectrode experiments acetylcholine (ACh), gastrin–cholecystokinin (CCK) as well as bombesin peptides evoked Ca2+-dependent opening of the K+ conductance pathway, resulting in membrane hyperpolarization. The large K+ channel, which is under strict dual control by internal Ca2+ and voltage, may provide a crucial link between hormone-evoked increase in internal Ca2+ concentration and the resulting NaCl-rich fluid secretion5,8.

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  1. Marty, A. Nature 291, 497–500 (1981).

    Article  ADS  CAS  Google Scholar 

  2. Pallotta, B. S., Magleby, K. L. & Barrett, J. N. Nature 293, 471–774 (1981).

    Article  ADS  CAS  Google Scholar 

  3. Latorre, R. & Miller, C. J. Membrane Biol. 71, 11–30 (1983).

    Article  CAS  Google Scholar 

  4. Meech, R. W. A. Rev. Biophys. Bioengng 7, 1–18 (1978).

    Article  ADS  CAS  Google Scholar 

  5. Maruyama, Y., Gallacher, D. V. & Petersen, O. H. Nature 302, 827–829 (1983).

    Article  ADS  CAS  Google Scholar 

  6. Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflügers Arch. ges. Physiol. 391, 85–100 (1981).

    Article  CAS  Google Scholar 

  7. Neher, E. in Techniques in Cellular Physiology Vol. 1, Pt II (ed. Baker, P. F.) P 121, 1–16 (Elsevier, Amsterdam, 1982).

    Google Scholar 

  8. Lindkaer-Jensen, S. et al. Acta physiol. scand. 111, 225–231 (1981).

    Article  CAS  Google Scholar 

  9. Petersen, O. H. The Electrophysiology of Gland Cells (Academic, London, 1980).

    Google Scholar 

  10. Ginsborg, B. L., House, C. R. & Silinsky, E. M. J. Physiol., Lond. 236, 723–731 (1974).

    Article  CAS  Google Scholar 

  11. Maruyama, Y. & Petersen, O. H. Nature 300, 61–63 (1982).

    Article  ADS  CAS  Google Scholar 

  12. O'Doherty, J. & Stark, R. J. Am. J. Physiol. 242, G513–521 (1982).

    CAS  PubMed  Google Scholar 

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Maruyama, Y., Petersen, O., Flanagan, P. et al. Quantification of Ca2+-activated K+ channels under hormonal control in pig pancreas acinar cells. Nature 305, 228–232 (1983).

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