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Construction of artificial chromosomes in yeast

Nature volume 305, pages 189–193 (1983)Cite this article

Abstract

Fifty-five-kilobase long artificial chromosomes containing cloned genes, replicators, centromeres and telomeres have been constructed in yeast. These molecules have many of the properties of natural yeast chromosomes. Centromere function is impaired on short (less than 20 kilobases) artificial chromosomes.

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Author information

Authors and Affiliations

  1. Dana–Farber Cancer Institute, 44 Binney St, Boston, Massachusetts, 02115, USA

    Andrew W. Murray & Jack W. Szostak

  2. Program in Cell and Developmental Biology, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Andrew W. Murray

  3. Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Jack W. Szostak

Authors
  1. Andrew W. Murray
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  2. Jack W. Szostak
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Murray, A., Szostak, J. Construction of artificial chromosomes in yeast. Nature 305, 189–193 (1983). https://doi.org/10.1038/305189a0

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