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Enhanced luminescence procedure for sensitive determination of peroxidase-labelled conjugates in immunoassay

Nature volume 305, pages 158–159 (1983)Cite this article

Abstract

Present luminescence assays for horseradish peroxidase (HRP) have limitations. Here we report a novel procedure in which the HRP-catalysed luminescence of a cyclic hydrazide (such as luminol) is multiplied severalfold by the addition of a synthetic component of the firefly bioluminescent system, D-luciferin (4,5-dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazole-carboxylic acid). The specific enhancement of HRP-catalysed light emission from cyclic hydrazides should extend the sensitivities of luminescently monitored assays, which have already been shown to be as sensitive as those using radioactive labels1,2. This procedure has been applied to the immunoassay of serum α-fetoprotein, thyroxine, digoxin, hepatitis B surface antigen, immunoglobulin E and rubella virus antibody.

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Authors and Affiliations

  1. Department of Clinical Chemistry, University of Birmingham, Birmingham, B15 2TT, UK

    Thomas P. Whitehead & Larry J. Kricka

  2. Department of Clinical Chemistry, Wolfson Research Laboratories, Queen Elizabeth Medical Centre, Birmingham, B15 2TH, UK

    Gary H. G. Thorpe, Timothy J. N. Carter & Carol Groucutt

Authors
  1. Thomas P. Whitehead
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  2. Gary H. G. Thorpe
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  3. Timothy J. N. Carter
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  4. Carol Groucutt
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  5. Larry J. Kricka
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Whitehead, T., Thorpe, G., Carter, T. et al. Enhanced luminescence procedure for sensitive determination of peroxidase-labelled conjugates in immunoassay. Nature 305, 158–159 (1983). https://doi.org/10.1038/305158a0

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