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Identification and chemical synthesis of a tandemly repeated immunogenic region of Plasmodium knowlesi circumsporozoite protein


Complementary DNA clones that code for the immunogenic region of the Plasmodium knowlesi circumsporozoite protein were shown to contain a tandemly repeating 36-base pair unit. A synthetic dodecapeptide corresponding to the predicted reading frame of the repeating nucleotide unit behaved, in an immunoradiometric assay, identically with the native P. knowlesi circumsporozoite protein. The repeating 36-base pair unit occurred 12 times within the gene and accounts for at least one-third of the amino acid sequence of the surface antigen protein.

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  1. Nussenzweig, R. S. Scand. J. infect. Dis. (Suppl.) 36, 40–45 (1983).

    Google Scholar 

  2. Cochrane, A., Santoro, F., Nussenzweig, V., Gwadz, R. W. & Nussenzweig, R. S. Proc. natn. Acad. Sci. U.S.A. 79, 5651–5655 (1982).

    Article  ADS  CAS  Google Scholar 

  3. Hollingdale, M. R., Zavala, F., Nussenzweig, R. & Nussenzweig, V. J. Immun. 128, 1929–1930 (1982).

    CAS  PubMed  Google Scholar 

  4. Ellis, J. et al. Nature 302, 536–538 (1983).

    Article  ADS  CAS  Google Scholar 

  5. Messing, J. & Vieira, J. Gene 19, 269–276 (1982).

    Article  CAS  Google Scholar 

  6. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  7. Godson, G. N. in Methods in Recombinant DNA (ed. Weissman, S.) (Praeger, New York, in the press).

  8. Lupski, J. R., Ozaki, L. S., Ellis, J. & Godson, G. N. Science 220, 1285–1288 (1983).

    Article  ADS  CAS  Google Scholar 

  9. Maizels, N. Nature 249, 647 (1974).

    Article  ADS  CAS  Google Scholar 

  10. Vieira, J. & Messing, J. Gene 19, 259–268 (1982).

    Article  CAS  Google Scholar 

  11. Powers, J. C., Gupton, B. F., Harley, A. D., Nishino, N. & Whitley, R. J. Biochim. biophys. Acta 485, 156–166 (1977).

    Article  CAS  Google Scholar 

  12. Zavala, F., Gwadz, R. W., Collins, F. H., Nussenzweig, R. S. & Nussenzweig, V. Nature 299, 737–738 (1982).

    Article  ADS  CAS  Google Scholar 

  13. Marglin, H. & Merrifield, R. B. A. Rev. Biochem. 39, 841–866 (1970).

    Article  CAS  Google Scholar 

  14. Merrifield, R. B. J. Am. chem. Soc. 85, 2149 (1963).

    Article  CAS  Google Scholar 

  15. Zavala, F., Cochrane, A. H., Nardin, E., Nussenzweig, R. S. & Nussenzweig, V. J. exp. Med. 157, 1947–1957 (1983).

    Article  CAS  Google Scholar 

  16. Vandenberg, J., Nussenzweig, R. S. & Most, H. Mil. Med. 134 (Suppl.), 1183–1190 (1969).

    Article  Google Scholar 

  17. Doolittle, R. F. in The Evolution of Proteins (eds Neurath, H. & Hill, R. L.) 1–18 (Academic, New York, 1979).

    Book  Google Scholar 

  18. Pedersen, K., Devereux, J., Wilson, D. R., Sheldon, E. & Larkins, B. A. Cell 29, 1015–1026 (1982).

    Article  CAS  Google Scholar 

  19. Beachey, E. H., Seyer, J. M. & Kang, A. H. J. biol. Chem. 255, 6284–6289 (1980).

    CAS  PubMed  Google Scholar 

  20. Sutcliffe, J. G. Cold Spring Harb. Symp. quant. Biol. 43, 77–90 (1979).

    Article  CAS  Google Scholar 

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Godson, G., Ellis, J., Svec, P. et al. Identification and chemical synthesis of a tandemly repeated immunogenic region of Plasmodium knowlesi circumsporozoite protein. Nature 305, 29–33 (1983).

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