Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Structure of the Ki-ras gene of the human lung carcinoma cell line Calu-1

Abstract

The homologue of the viral Kirsten ras (ν-Ki-ras) gene found in the human lung carcinoma cell line, Calu-1, has an intron–exon structure similar to that of the human homologue of the viral Harvey ras (v-Ha-ras) gene. A second, potential fourth coding exon is present in the human Ki-ras gene and similar sequences are found in the Kirsten murine sarcoma virus. Cysteine is encoded at the twelfth amino acid position, suggesting that the Calu-1 Ki-ras gene has undergone a mutational activation at the same position as the human Ha-ras gene of the bladder carcinoma cell line, T24. A comparison of their predicted amino acid sequences suggests that ras proteins have a ‘constant’ region and a ‘variable’ region. Here we propose a common modular structure for ras gene products in which the variable region forms a physiologically important combining site.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

References

  1. Ellis, R. et al. Nature 292, 506–511 (1981).

    ADS  CAS  Article  PubMed  Google Scholar 

  2. Der, C., Krontiris, T. & Cooper, G. Proc. natn. Acad. Sci. U.S.A. 79, 3637–3640 (1982).

    ADS  CAS  Article  Google Scholar 

  3. Parada, L., Tabin, C., Shih, C. & Weinberg, R. Nature 297, 474–478 (1982).

    ADS  CAS  Article  PubMed  Google Scholar 

  4. Santos, E., Tronick, S., Aaronson, S., Pulciani, S. & Barbacid, M. Nature 298, 343–347 (1982).

    ADS  CAS  Article  PubMed  Google Scholar 

  5. Shimizu, K. et al. Proc. natn. Acad. Sci. U.S.A. 80, 2112–2116 (1983).

    ADS  CAS  Article  Google Scholar 

  6. Taparowsky, E. et al. Nature 300, 762–765 (1982).

    ADS  CAS  Article  PubMed  Google Scholar 

  7. Reddy, E., Reynolds, R., Santos, E. & Barbacid, M. Nature 300, 149–152 (1982).

    ADS  CAS  Article  PubMed  Google Scholar 

  8. Tabin, C. et al. Nature 300, 143–148 (1982).

    ADS  CAS  Article  PubMed  Google Scholar 

  9. Perucho, M. et al. Cell 27, 467–476 (1981).

    CAS  Article  PubMed  Google Scholar 

  10. Tsuchida, N., Ryder, T. & Ohtsubo, E. Science 217, 937–939 (1982).

    ADS  CAS  Article  PubMed  Google Scholar 

  11. Fasano, O., Taparowsky, E., Fiddes, J., Wigler, M. & Goldfarb, M. J. molec. appl. Genet. (in the press).

  12. Capon, D., Chen, E., Levinson, A., Seeburg, P. & Goeddel, D. Nature 302, 33–37 (1983).

    ADS  CAS  Article  PubMed  Google Scholar 

  13. Alt, F. et al. Cell 20, 293–301 (1980).

    CAS  Article  PubMed  Google Scholar 

  14. Early, P. et al. Cell 20, 313–319 (1980).

    CAS  Article  PubMed  Google Scholar 

  15. Amara, S., Jonas, V., Rosenfeld, M., Ong, E. & Evans, R. Nature 298, 240–244 (1982).

    ADS  CAS  Article  PubMed  Google Scholar 

  16. Ullrich, A. et al. Science 196, 1313–1319 (1977).

    ADS  CAS  Article  PubMed  Google Scholar 

  17. Perler, F. et al. Cell 20, 555–566 (1980).

    CAS  Article  PubMed  Google Scholar 

  18. Rice, C. & Strauss, J. Proc. natn. Acad. Sci. U.S.A. 78, 2062–2066 (1981).

    ADS  CAS  Article  Google Scholar 

  19. Shih, T. et al. J. Virol. 42, 253–261 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  20. Chang, E., Gonda, M., Ellis, R., Scolnick, E. & Lowy, D. Proc. natn. Acad. Sci. U.S.A. 79, 4848–4852 (1982).

    ADS  CAS  Article  Google Scholar 

  21. Maniatis, T., Jeffrey, A. & Kleid, D. Proc. natn. Acad. Sci. U.S.A. 72, 1184–1188 (1975).

    ADS  CAS  Article  Google Scholar 

  22. Maxam, A. & Gilbert, W. Meth. Enzym. 65, 499–580 (1980).

    CAS  Article  PubMed  Google Scholar 

  23. Drouin, J. J. molec. Biol. 140, 15–34 (1980).

    CAS  Article  PubMed  Google Scholar 

  24. Dahr, R. et al. Science 217, 934–937 (1982).

    ADS  Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Shimizu, K., Birnbaum, D., Ruley, M. et al. Structure of the Ki-ras gene of the human lung carcinoma cell line Calu-1. Nature 304, 497–500 (1983). https://doi.org/10.1038/304497a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/304497a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing