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A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation

Nature volume 304, pages 184–187 (1983)Cite this article

Abstract

The T-DNA region of Agrobacterium tumefaciens tumour-inducing plasmids of the nopaline type1 contains a gene coding for the enzyme nopaline synthase. This gene is expressed constitutively in host plant cells to which it is transferred during tumour induction2. We have exploited the regulatory elements of this gene to construct a chimaeric gene that confers antibiotic resistance on transformed plant cells. The chimaeric gene encodes the expected chimaeric transcripts in plant cells, and confers on transformed cells the ability to grow in the presence of normally lethal levels of the antibiotic G418 (ref. 3). Experiments using in vitro transformation techniques on single plant cells indicate that this antibiotic resistance can be used as a selectable marker, and can therefore be used in selecting cells transformed by T-DNA vectors that have had the genes for hormone autotrophy deleted4. Plant cells transformed by such ‘disarmed’ T-DNA vectors can be regenerated into entire plants, whose sexual progeny contain unaltered copies of the inciting T-DNA5. The availability of this dominant selectable marker should allow a wider range of experiments to be under taken using different host plants.

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References

  1. Leemans, J. et al. J. molec. appl. Genet. 1, 149–164 (1981).

    CAS  Google Scholar 

  2. Wullems, G. J., Molendijk, L., Ooms, G. & Schilperoort, R. A. Cell 24, 719–727 (1981).

    Article  CAS  Google Scholar 

  3. Colbere-Garapin, F., Horodniceanu, F., Kourilsky, P. & Garapin, A.-C. J. molec. Biol. 150, 1–14 (1981).

    Article  CAS  Google Scholar 

  4. Leemans, J. et al. J. molec. appl. Genet. 1, 149–164 (1981).

    CAS  Google Scholar 

  5. Barton, K. A., Binns, A. N., Matzke, A. J. M. & Chilton, M.-D. Cell 32, 1033–1043 (1983).

    Article  CAS  Google Scholar 

  6. Depicker, A., Stachel, S., Dhaese, P., Zambryski, P. & Goodman, H. M. J. molec. appl. Genet. 1, 561–573 (1982).

    CAS  Google Scholar 

  7. Bevan, M., Barnes, W. M. & Chilton, M.-D. Nucleic Acids Res. 11, 369–385 (1983).

    Article  CAS  Google Scholar 

  8. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  9. Messing, J., Crea, R. & Seeburg, P. H. Nucleic Acids Res. 9, 309–323 (1981).

    Article  CAS  Google Scholar 

  10. Matzke, A. J. M. & Chilton, M.-D. J. molec. appl. Genet. 1, 39–49 (1981).

    CAS  Google Scholar 

  11. Oka, A., Sugisaki, H. & Takanami, M. J. molec. Biol. 147, 217–226 (1981).

    Article  CAS  Google Scholar 

  12. de Framond, A. J., Barton, K. A. & Chilton, M.-D. Bio/Technology 1, 262–269 (1983).

    Google Scholar 

  13. Rothstein, S. J., Jorgenson, R. A., Postle, K. & Reznikoff, W. S. Cell 19, 795–805 (1980).

    Article  CAS  Google Scholar 

  14. Auerswald, E. A., Ludwig, G. & Schaller, H. Cold Spring Harb. Symp. quant. Biol. 45, 107–113 (1980).

    Article  Google Scholar 

  15. Holsters, M. et al. Molec. gen. Genet. 163, 181–187 (1978).

    Article  CAS  Google Scholar 

  16. Murashige, T. & Skoog, F. Pl. Physiol. 15, 473–497 (1962).

    Article  CAS  Google Scholar 

  17. Meins, F. & Binns, A. Proc. natn. Acad. Sci. U.S.A. 74, 2928–2932 (1977).

    Article  ADS  Google Scholar 

  18. Chilton, M.-D. et al. Nature 295, 432–434 (1982).

    Article  ADS  CAS  Google Scholar 

  19. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  20. Maniatis, T., Jeffrey, A. & Kleid, D. G. Proc. natn. Acad. sci. U.S.A. 72, 1184–1188 (1975).

    Article  ADS  CAS  Google Scholar 

  21. Depicker, A. et al. Plasmid 3, 193–211 (1980).

    Article  CAS  Google Scholar 

  22. Rave, N., Ckvenjakov, R. & Boedtker, H. Nucleic Acids Res. 6, 3559–3567 (1979).

    Article  CAS  Google Scholar 

  23. Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1981).

    Article  ADS  Google Scholar 

  24. Bevan, M. W. & Chilton, M.-D. J. molec. appl. Genet. 1, 539–546 (1982).

    CAS  Google Scholar 

  25. Wullems, G. J., Molendijk, L., Ooms, G. & Schilperoort, R. A. Proc. natn. Acad. Sci. U.S.A. 78, 4344–4348 (1981).

    Article  ADS  CAS  Google Scholar 

  26. Green, M. R. & Roeder, R. G. Cell 22, 231–242 (1980).

    Article  CAS  Google Scholar 

  27. Bolivar, F. Gene 4, 121–136 (1978).

    Article  CAS  Google Scholar 

  28. Ditta, G., Stansfield, S., Corbin, D. & Helinski, D. Proc. natn. Acad. Sci. U.S.A. 77, 7347–7351 (1980).

    Article  ADS  CAS  Google Scholar 

  29. Thomashow, M. F. et al. Proc. natn. Acad. Sci. U.S.A. 77, 6448–6452 (1980).

    Article  ADS  CAS  Google Scholar 

  30. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  31. Herrera-Estrella, L., Depicker, A., Van Montagu, M. & Schell, J. Nature 303, 209–213 (1983).

    Article  ADS  CAS  Google Scholar 

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Authors and Affiliations

  1. Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, CB2 2LQ, UK

    Michael W. Bevan & Richard B. Flavell

  2. Department of Biology, Washington University, St Louis, Missouri, 63130, USA

    Mary-Dell Chilton

Authors
  1. Michael W. Bevan
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  2. Richard B. Flavell
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  3. Mary-Dell Chilton
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Bevan, M., Flavell, R. & Chilton, MD. A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation. Nature 304, 184–187 (1983). https://doi.org/10.1038/304184a0

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