B lymphocytes originate from pluripotential haematopoietic stem cells and differentiate into immunoglobulin (Ig)-producing cells. B-cell lineage differentiation is accompanied by two types of immunoglobulin gene rearrangements1–3—rearrangement of V, D and J gene segments to create a functional V gene and rearrangement of CH genes for heavy-chain switching. These results, however, have been obtained mainly by analysis of immunoglobulin gene organization of myeloma cells. Baltimore and his colleagues have established Abelson murine leukaemia virus (A-MuLV)-transformed cell lines4 and found a few lines capable of carrying out κ-gene rearrangement5 or undergoing isotype switching during in vitro culture6. To study early B-cell lineage differentiation events, we have now also established A-MuLV-transformed cell lines which are capable of differentiating from μ− to μ+and of undergoing continuing rearrangement of heavy-chain genes in culture. Analysis of immunoglobulin gene organization of these transformed cells revealed that μ− cells have already undergone DNA rearrangements involving JH segments but an additional rearrangement of JH segments is required for initiation of μ-chain synthesis. Southern blot analysis of the DNA and two-dimensional gel electrophoresis of intracytoplasmic μ-chain show that μ-chain diversity with respect to antigen specificity may be generated during this second rearrangement process. As no rearrangement of light-chain genes takes place in these cells, this implies that light-chain gene rearrangement requires some further change, or a different enzyme.
Access optionsAccess options
Subscribe to Journal
Get full journal access for 1 year
only $3.90 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Sakano, H., Maki, R., Kurosawa, Y., Reeder, W. & Tonegawa, S. Nature 286, 676–683 (1980).
Early, P., Huang, H., Davis, M., Calame, K. & Hood, L. Cell 19, 981–992 (1980).
Honjo, T. & Kataoka, T. Proc. natn. Acad. Sci. U.S.A. 75, 2140–2144 (1978).
Alt, F., Rosenberg, N., Lewis, S., Thomas, E. & Baltimore, D. Cell 27, 381–390 (1981).
Lewis, S., Rosenberg, N., Alt, F. & Baltimore, D. Cell 30, 807–816 (1982).
Alt, F., Rosenberg, N., Casanova, R. J., Thomas, E. & Baltimore, D. Nature 296, 325–331 (1982).
Sugiyama, H. et al. J. Immun. 126, 2793–2797 (1982).
Whitlock, C. A., Ziegler, S. F., Treiman, L. Z., Stafford, J. I. & Witte, O. N. Cell 32, 903–911 (1983).
Nottenberg, C. & Weissman, I. Proc. natn. Acad. Sci. U.S.A. 78, 484–488 (1981).
Burrows, P. D., Lejeune, M. & Kearney, J. F. Nature 280, 838–841 (1979).
Maki, R., Kearney, J. F., Paige, C. & Tonegawa, S. Science 209, 1366–1369 (1980).
Perry, R. P., Kelley, D. E., Coleclough, C. & Kearney, J. F. Proc. natn. Acad. Sci. U.S.A. 78, 247–251 (1981).
Korsmeyer, S. J. et al. Proc. natn. Acad. Sci. U.S.A. 78, 7096–7100 (1981).
Sakano, H., Kurosawa, Y., Weigert, M. & Tonegawa, S. Nature 290, 562–565 (1981).
Sieberlist, U., Ravetch, J. V., Korsmeyer, S., Waldmann, T. & Leder, P. Nature 294, 631–635 (1981).
Kurosawa, Y. et al. Nature 290, 565–570 (1981).
About this article
DELETION OF THE 3 ' SPLICE SITE OF THE LEADER-VARIABLE REGION INTRON OF IMMUNOGLOBULIN HEAVY CHAIN GENES INDUCES A DIRECT SPLICING OF LEADER TO CONSTANT REGION, RESULTING IN THE PRODUCTION OF TRUNCATED ?-CHAINS
European Journal of Immunogenetics (1995)
Leukemia & Lymphoma (1995)
European Journal of Immunology (1993)
N sequences, P nucleotides and short sequence homologies at junctional sites in VH to VHDJH and VHDJH to JH joining
Molecular Immunology (1993)
Nucleotide deletion of T cell receptor Vγ2 and Jγ2 coding sequences at Vγ2−Jγ2 junctions in immature B cell lines
Molecular Immunology (1992)