Abstract
Foreign genes introduced into plant cells with Ti-plasmid vectors are not expressed. We have constructed an expression vector derived from the promoter sequence of nopaline synthase, and have inserted the coding sequences of the octopine synthase gene and a chloramphenicol acetyltransferase gene into this vector. These chimaeric genes are functionally expressed in plant cells after their transfer via a Ti-plasmid of Agrobacterium tumefaciens.
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References
Bevan, M. W. & Chilton, M.-D. A. Rev. Genet. 16, 357–384 (1982).
Ream, L. & Gordon, M. P. Science 218, 854–858 (1982).
Van Montagu, M. & Schell, J. Curr. Topics Microbiol. Immun. 96, 237–245 (1981).
Schell, J. et al. Cell Fusion (Raven, New York, in the press).
Hernalsteens, J. P. et al. Nature 287, 654–656 (1980).
Drummond, M. H., Gordon, M. P., Nester, E. W. & Chilton, M.-D. Nature 269, 535–536 (1977).
Willmitzer, L., Simons, G. & Schell, J. EMBO J. 1, 139–146 (1982).
Willmitzer, L. et al. Cell 32, 1045–1056 (1983).
Bevan, M. W. & Chilton, M.-D. J. molec. appl. Genet. 1, 539–546 (1982).
Gelvin, S. B., Gordon, M. P., Nester, E. W. & Aronson, A. I. Plasmid 6, 17–29 (1981).
Garfinkel, D. J. et al. Cell 27, 143–153 (1981).
Leemans, J. et al. EMBO J. 1, 147–152 (1982).
Joos, H. et al. Cell 32, 1057–1067 (1983).
De Greve, H. et al. J. molec. appl. Genet. 1, 499–512 (1982).
Depicker, A., Stachel, S., Dhaese, P., Zambryski, P. & Goodman, H. M. J. molec. appl. Genet. 1, 561–574 (1982).
Bevan, M., Barnes, W. M. & Chilton, M.-D. Nucleic Acids Res. 11, 369–385 (1983).
Breathnach, R. & Chambon, P. A. Rev. Biochem. 50, 349–383 (1981).
Fitzgerald, M. & Shenk, T. Cell 24, 251–260 (1981).
Dhaese, P. et al. EMBO J. 2, 419–426 (1983).
De Greve, H. et al. Nature 300, 752–755 (1982).
Otten, L. et al. Molec. gen. Genet. 183, 209–213 (1981).
Otten, L. Pl. Sci. Lett. 25, 15–27 (1982).
Aerts, M., Jacobs, M., Hernalsteens, J. P., Van Montagu, M. & Schell, J. Pl. Sci. Lett. 17, 43–50 (1979).
Leemans, J. et al. J. molec. appl. Genet. 1, 149–164 (1981).
Rao, R. N. & Rogers, S. G. Gene 7, 79–82 (1979).
Kozak, M. Nucleic Acids Res. 9, 5233–5252 (1981).
Lomedico, P. T. & McAndrew, S. J. Nature 299, 221–226 (1982).
Remaut, E., Stanssens, P. & Fiers, W. Gene 15, 81–93 (1981).
Van Haute, E. et al. EMBO J. 2, 411–418 (1983).
Leemans, J. et al. Gene 19, 361–364 (1982).
Zambryski, P., Depicker, A., Kruger, K., Goodman, H. M. J. molec. appl. Genet. 1, 361–370 (1982).
Bolivar, F. Gene 4, 121–136 (1978).
Cohen, J. et al. Proc. natn. Acad. Sci. U.S.A. 77, 1078–1082 (1980).
Gorman, C. M., Moffat, L. F. & Howard, B. H. Molec. cell. Biol. 2, 1044–1051 (1982).
Marcoli, R., Iida, S. & Bickle, T. A. FEBS Lett. 110, 11–14 (1980).
Prentki, P., Karch, F., Iida, S. & Meyer, J. Gene 14, 289–299 (1981).
Zaidenzaig, Y., Fitton, J. E., Packman, L. C. & Shaw, W. V. Eur. J. Biochem. 100, 609–618 (1979).
Wöstemeyer, A. et al. Genetic Engineering in Eukaryotes (ed. Lurquin, P.) (Plenum, New York, in the press).
Beck, E., Ludwing, G., Averswald, E. A., Reiss, B. & Schaller, H. Gene 19, 329–336 (1982).
Fling, M. E. & Elwell, L. P. J. Bact. 141, 779–785 (1980).
Herrera-Estrella, L. et al. EMBO J. (in the press).
Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–559 (1980).
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Herrera-Estrella, L., Depicker, A., Van Montagu, M. et al. Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector. Nature 303, 209–213 (1983). https://doi.org/10.1038/303209a0
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DOI: https://doi.org/10.1038/303209a0
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