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Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector

Nature volume 303, pages 209213 (19 May 1983) | Download Citation

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Abstract

Foreign genes introduced into plant cells with Ti-plasmid vectors are not expressed. We have constructed an expression vector derived from the promoter sequence of nopaline synthase, and have inserted the coding sequences of the octopine synthase gene and a chloramphenicol acetyltransferase gene into this vector. These chimaeric genes are functionally expressed in plant cells after their transfer via a Ti-plasmid of Agrobacterium tumefaciens.

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Author information

Affiliations

  1. Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium

    • Luis Herrera-Estrella
    • , Ann Depicker
    • , Marc Van Montagu
    •  & Jeff Schell
  2. Max-Planck-Institut für Züchtungsforschung, D-5000 Köln 30, FRG

    • Jeff Schell

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https://doi.org/10.1038/303209a0

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