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A secreted phosphoprotein marker for neoplastic transformation of both epithelial and fibroblastic cells

Naturevolume 302pages714715 (1983) | Download Citation

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Abstract

A wide variety of virally and spontaneously transformed fibroblasts secrete a major transformation-related phosphoprotein with a molecular weight (MW), depending on the species of origin, of about 62,000 (62K)1–13. Markedly elevated extracellular levels of this major 32P-labelled protein are not simply linked to exponential growth1 but instead are associated directly with transformation. The phosphoprotein is not antigenically related to p60src, p60c-src or simian virus 40 (SV40) non-viral T antigen2, and it is further distinguishable from SV40 non-viral T antigen (pp 53) on the basis of its electrophoretic mobility. In this study we have compared a variety of normal and transformed epithelial cells for secretion of this 32P-labelled protein and have found that this marker distinguishes neoplastic from preneoplastic and normal mouse mammary epithelium and also identifies highly tumorigenic cells derived from guinea pig bile duct epithelium and rat liver epithelium. Because the classical phenotypic properties commonly associated with transformation of fibroblasts cannot be generally used to discriminate tumorigenic from non-tumorigenic epithelial cells4–9, this phosphoprotein, which identifies tumorigenic cells of both fibroblastic and epithelial origin, is likely to be of particular importance.

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References

  1. 1

    Senger, D. R., Wirth, D. F. & Hynes, R. O. Cell 16, 885–893 (1979).

  2. 2

    Senger, D. R., Wirth, D. F., Bryant, C. & Hynes, R. O. Cold Spring Harb. Symp. quant. Biol. 44, 651–657 (1980).

  3. 3

    Senger, D. R., Wirth, D. F. & Hynes, R. O. Nature 286, 619–621 (1980).

  4. 4

    Butel, J. S., Dudley, J. P. & Noonan, C. P. in Cell Biology of Breast Cancer (eds McGrath, C., Brennan, M. S. & Rich, M. A.) 317–345 (Academic, New York, 1980).

  5. 5

    Hosick, H. L., Anderson, L. W., Angello, J. C. & Danielson, K. G. in Cell Biology of Breast Cancer (eds McGrath, C., Brennan, M. S. & Rich, M. A.) 393–412 (Academic, New York, 1980).

  6. 6

    Miller, F. R., Dexter, D. L. & Heppner, G. H. in Cell Biology of Breast Cancer (eds McGrath, C., Brennan, M. S. & Rich, M. A.) 413–422 (Academic, New York, 1980).

  7. 7

    Marshall, C. J., Franks, L. M. & Carbonell, A. W. J. natn. Cancer Inst. 58, 1743–1751 (1977).

  8. 8

    San, R. H. C. et al. Cancer Res. 39, 1026–1034 (1979).

  9. 9

    Yuspa, S. H., Hahley-Nelson, P., Koehler, B. & Stanley, J. R. Cancer Res. 40, 4694–4703 (1980).

  10. 10

    Asch, B. B. & Medina, D. J. natn. Cancer Inst. 61, 1423–1430 (1978).

  11. 11

    Medina, D. & DeOme, K. B. J. natn. Cancer Inst. 45, 353–363 (1970).

  12. 12

    Asch, B. B., Medina, D. & Brinkley, B. R. Cancer Res. 39, 893–907 (1979).

  13. 13

    Laemmli, U. K. Nature 227, 680–685 (1970).

  14. 14

    Kessler, S. W. J. Immun. 115, 1617–1624 (1975).

  15. 15

    Zbar, B., Wepsic, H. T., Rapp, H. J., Whang-Peng, J. & Borsos, T. J. natn. Cancer Inst. 43, 821–831 (1969).

  16. 16

    Zbar, B., Bernstein, I., Tanaka, T. & Rapp, H. J. Science 170, 1217–1218 (1970).

  17. 17

    Dvorak, H. F., Dvorak, A. M., Manseau, E. J., Wiberg, L. & Churchill, W. H. J. natn. Cancer Inst. 62, 1459–1472 (1979).

  18. 18

    Hunter, J. T., Ashley, M. P., Rapp, H. J., Sukumar, S. & Zbar, B. Cancer Immun. Immunother. 11, 39–44 (1981).

  19. 19

    Weinstein, I. B., Orenstein, J. M., Gebart, R., Kaighn, M.E. & Stadier, U. C. Cancer Res. 35, 253–263 (1975).

  20. 20

    Yamaguchi, N. & Weinstein, I. B. Proc. natn. Acad. Sci. U.S.A. 72, 214–218 (1975).

  21. 21

    Cooper, J. A. & Hunter, T. Molec. Cell. Biol. 1, 165–178 (1981).

  22. 22

    Otto, G., Baltimore, D. & Hynes, R. O. J. Virol. (submitted).

  23. 23

    Hunter, T. & Sefton, B. Proc. natn. Acad. Sci. U.S.A. 77, 1311–1315 (1980).

  24. 24

    Witte, O. N., Dasgupta, A. & Baltimore, D. Nature 283, 826–831 (1980).

  25. 25

    Feldman, R. A., Hanafusa, T. & Hanafusa, H. Cell 22, 757–766 (1980).

  26. 26

    Das, N. K., Hosick, H. L. & Nandi, S. J. natn. Cancer Inst. 52, 849–861 (1974).

  27. 27

    Pickett, P. B., Pitelka, D. R., Hamamoto, S. T. & Misfeldt, D. S. J. Cell Biol. 66, 316–332 (1975).

  28. 28

    Asch, B. B., Medina, D., Kretzer, F., Connolly, J. L. & Brinkley, B. R. Cancer Res. 40, 2383–2389 (1980).

  29. 29

    McGrath, C. M., Maloney, T. & Soule, H. D. in Cell Biology of Breast Cancer (eds McGrath, C., Brennan, M. S. & Rich, M. A.) 373–391 (Academic, New York, 1980).

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Affiliations

  1. Department of Pathology, Beth Israel Hospital and Harvard Medical School, Beth Israel Hospital, Boston, Massachusetts, 02215, USA

    • Donald R. Senger
    • , Carole A. Perruzzi
    •  & Harold F. Dvorak
  2. Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, New York, 14263, USA

    • Bonnie B. Asch
  3. Veterans Administration Outpatient Clinic and Boston University School of Medicine, 17 Court St, Boston, Massachusetts, 02108, USA

    • Barbara D. Smith
  4. the Charles A. Dana Research Institute, Beth Israel Hospital, Boston, Massachusetts, 02215, USA

    • Donald R. Senger
    • , Carole A. Perruzzi
    •  & Harold F. Dvorak

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https://doi.org/10.1038/302714a0

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