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Three-dimensional architecture of a polytene nucleus

Naturevolume 302pages676681 (1983) | Download Citation

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Abstract

The three-dimensional chromosome topography in an intact nucleus has been determined using fluorescently stained Drosophila polytene chromosomes, optical fluorescence microscopy and newly developed, generally applicable, cellular image reconstruction techniques. The folding pattern is a complex mixture of parallel chromosomal segments and intertwined coils and shows extensive interaction of the chromosomes with the nuclear envelope.

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  1. Department of Biochemistry and Biophysics, University of California, San Francisco, California, 94143, USA

    • David A. Agard
    •  & John W. Sedat

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https://doi.org/10.1038/302676a0

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