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Nucleotide sequence of cassava latent virus DNA

Nature volume 301, pages 260262 (20 January 1983) | Download Citation

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Abstract

Only two groups of plant viruses, the caulimoviruses1,2 and the geminiviruses3, are known to contain a genome of DNA. Unlike that of the caulimoviruses, the genome of the geminivinises is composed of single-stranded, covalently-closed circles of DNA. There is evidence that the geminiviruses, specifically bean golden mosaic virus4 and tomato golden mosaic virus5, have a genome composed of two similar-sized circles of DNA, and the molecular cloning of both components from tomato golden mosaic virus has recently been reported6. No information is available, however, concerning the protein coding capacity of the genome, the possible modes of UNA transcription and DNA replication and the assembly of mature components into the characteristic geminate particles. We report here on the derivation of the nucleotide sequence of the geminivirus, cassava latent virus (CLV), as a fundamental step towards the investigation of the mode of replication of this group of viruses. Results show that CLV DNA comprises two similar-sized molecular species with a common region encompassing almost 200 nucleotides. The organization of the genome is discussed.

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Author information

Affiliations

  1. Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, UK

    • John Stanley
    •  & Michael R. Gay

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DOI

https://doi.org/10.1038/301260a0

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