Abstract
The pituitary neurointermediate lobe of genetically obese (ob/ob) mice contains a hormone which stimulates insulin release1–3 and which cross-reacts with a -COOH-terminal ACTH antiserum, suggesting that it is related to corticotropin-like intermediate lobe peptide (CLIP), the 18–39 fragment of ACTH4–6. The hormone, which we have called β-cell-tropin7, has been shown to be present in the plasma of the ob/ob mouse8 and to potentiate glucose induced insulin secretion9. We have now shown that ACTH22–39 prepared by tryptic digestion of human synthetic CLIP behaves similarly on Biogel chromatography and on reverse-phase HPLC to the naturally occurring β-cell-tropin. Furthermore, β-cell-tropin and ACTH22–39 have indistinguishable antigenic and insulin releasing properties.
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Beloff-Chain, A., Morton, J., Dunmore, S. et al. Evidence that the insulin secretagogue, β-cell-tropin, is ACTH22–39. Nature 301, 255–258 (1983). https://doi.org/10.1038/301255a0
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DOI: https://doi.org/10.1038/301255a0
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