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Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli

Nature volume 301, pages 214221 (20 January 1983) | Download Citation

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Abstract

Bacterial clones containing human tissue-type plasminogen activator (t-PA) cDNA sequences were identified in a cDNA library prepared using gel-fractionated mRNA from human melanoma cells. A plasmid containing the Escherichia coli trp promoter and the cDNA sequence coding f or the 527-amino acid mature t-PA protein was constructed for expression in E. coli. A polypeptide was produced having the fibrinolytic properties characteristic of authentic human t-PA.

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References

  1. 1.

    Thromb. Haemostasis 43, 77–89 (1980).

  2. 2.

    & Nature 272, 549–550 (1978).

  3. 3.

    in Fibrinolysis (eds Kline, D. L. & Reddy, K. N. N.) 185–200 (CRC, Boca Raton, Florida, 1980).

  4. 4.

    in Fibrinolytics and Antifibrinolytics (ed. Markwardt, F.) 209–237 (Springer, New York, 1978).

  5. 5.

    in Fibrinolytics and Antifibrinolytics (ed. Markwardt, F.) 179–207 (Springer, New York, 1978).

  6. 6.

    in Proteases and Biological Control (eds Reich, E., Rifkin, D. B. & Shaw, E.) 333–341 (Cold Spring Harbor Laboratory, New York, 1975).

  7. 7.

    & J. biol. Chem. 256, 7035–7041 (1981).

  8. 8.

    , & Thromb. Haemostasis 45, 225–229 (1981).

  9. 9.

    & Thromb. Haemostasis 46, 662–665 (1981).

  10. 10.

    & Thromb. Haemostasis 46, 561–565 (1981).

  11. 11.

    , , & J. biol. Chem. 257, 2912–2919 (1982).

  12. 12.

    , & Thromb. Haemostasis 46, 658–661 (1981).

  13. 13.

    , & Nature 291, 590–591 (1981).

  14. 14.

    , , , & J. clin. Invest. 69, 573–580 (1982).

  15. 15.

    et al. Lancet ii, 1018–1020 (1981).

  16. 16.

    , , & J. biol. Chem. 252, 1102–1106 (1978).

  17. 17.

    , & Virology 108, 47–70 (1981).

  18. 18.

    & Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

  19. 19.

    , , & Biochemistry 16, 4743–4751 (1977).

  20. 20.

    , , & Virology 98, 251–254 (1979).

  21. 21.

    et al. Nature 295, 503–508 (1982).

  22. 22.

    & Eur. J. Biochem. 67, 247–256 (1976).

  23. 23.

    & J. Cell Biol. 67, 852–862 (1975).

  24. 24.

    , & J. biol. Chem. 253, 2483–2495 (1978).

  25. 25.

    et al. Nature 281, 544–548 (1979).

  26. 26.

    , & Proc. natn. Acad. Sci. U.S.A. 73, 4174–4178 (1976).

  27. 27.

    & Nucleic Acids Res. 8, 2331–2348 (1980).

  28. 28.

    & Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).

  29. 29.

    & Nucleic Acids Res. 7, 1513–1523 (1979).

  30. 30.

    Meth. Enzym. 65, 560–580 (1980).

  31. 31.

    , & Nucleic Acids Res. 9, 309–321 (1981).

  32. 32.

    & Meth. Enzym. 65, 499–560 (1980).

  33. 33.

    et al. Nucleic Acids Res 9, 6103–6114 (1981).

  34. 34.

    , , , & Cell 15, 1157–1174 (1978).

  35. 35.

    J. molec. Biol. 98, 503–517 (1975).

  36. 36.

    , & Biochim. biophys. Acta 442, 324–330 (1976).

  37. 37.

    & Science 196, 180–182 (1977).

  38. 38.

    et al. Cell 15, 687–701 (1978).

  39. 39.

    , & Nucleic Acids Res. 7, 1541–1552 (1979).

  40. 40.

    et al. Nature 287, 411–416 (1980).

  41. 41.

    et al. Symp. Soc. exp. Med. 33, 9–36 (1979).

  42. 42.

    & Biochem. biophys. Res. Commun. 74, 1220–1226 (1977).

  43. 43.

    & Nature 263, 211–214 (1976).

  44. 44.

    in Hormonal Proteins and Peptides (ed. Li, C. H.) 1–15 (Academic, New York, 1973).

  45. 45.

    & J. exp. Med. 148, 223–234 (1978).

  46. 46.

    & in Hemostasis and Thrombosis: Basic Principles and Clinical Practice, 1028–1036 (Lippincott, Philadelphia, 1982).

  47. 47.

    & Biochim. biophys. Acta 221, 20–30 (1970).

  48. 48.

    & Biochim. biophys. Acta 257, 122–234 (1972).

  49. 49.

    , , , & in Progress in Chemical Fibrinolysis and Thrombolysis Vol. 3 (eds Davidson, J. F., Rowan, R. M., Samana, M. M. & Desnoyers, P.C.) 191–209 (Raven, New York, 1972).

  50. 50.

    , , , & Proc. natn. Acad. Sci. U.S.A. 72, 2577–2581 (1975).

  51. 51.

    , , , & in Proteolysis and Physiological Regulation (eds Ribbons, D. W. & Brew, K.) 203–212 (Academic, New York, 1976).

  52. 52.

    , , & in Proteases and Biological Control (eds Reich, E., Rifkin, D. B. & Shaw, E.) 123–149 (Cold Spring Harbor Laboratory, New York, 1975).

  53. 53.

    et al. Proc. 4th int. Symp. on Genetics of Industrial Microorganisms June 1982, Kyoto, Japan (in the press).

  54. 54.

    Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3 (National Biomedical Research Foundation, Washington DC, 1978).

  55. 55.

    Nature 227, 680–685 (1970).

  56. 56.

    & Eur. J. Biochem. 46, 83–88 (1974).

  57. 57.

    , , & J. Virol. 9, 61–69 (1972).

  58. 58.

    & J. molec. Biol. 78, 321–335 (1973).

  59. 59.

    et al. Nature 275, 617–624 (1978).

  60. 60.

    et al. Gene 2, 95–113 (1977).

  61. 61.

    Experiments in Molecular Genetics, 431–433 (Cold Spring Harbor Laboratory, New York, 1972).

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Affiliations

  1. Department of Molecular Biology, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California 94080, USA

    • Diane Pennica
    • , William E. Holmes
    • , Elizabeth Yelverton
    • , Peter H. Seeburg
    • , Herbert L. Heyneker
    •  & David V. Goeddel
  2. Department of Protein Biochemistry, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California 94080, USA

    • William J. Kohr
    • , Richard N. Harkins
    • , Gordon A. Vehar
    • , Carole A. Ward
    •  & William F. Bennett
  3. Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, B-3000 Leuven, Belgium

    • Desire Collen

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https://doi.org/10.1038/301214a0

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