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Electrostatic orientation during electron transfer between flavodoxin and cytochrome c

Nature volume 301, pages 169171 (13 January 1983) | Download Citation

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Abstract

Various studies have shown that reaction rates between revers-ibly binding electron transfer proteins depend strongly on solution ionic strength1–7. These observations suggest that inter-molecular electrostatic interactions are important in facilitating the formation of a productive reaction complex. A recently examined system involves the reduction of vertebrate cytochrome c by bacterial flavodoxin8,9. Although this is a non-physiological reaction, it proceeds with rates typical for natural partners and is similarly inhibited at high ionic strengths. Here we describe computational studies which examine the role of electrostatics in the formation of a putative reaction complex between flavodoxin and cytochrome c. The results suggest that electrostatic interactions preorient the molecules before they make physical contact, facilitating the formation of an optimal reaction complex.

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Author information

Author notes

    • James B. Matthew
    • , P. C. Weber
    •  & F. R. Salemme

    Present addresses: Department of Biochemistry, University of New Hampshire, Durham, New Hampshire 03824, USA (J.B.M.); Department of Biochemistry, University of Arizona, Tucson, Arizona 85721, USA (P.C.W., F.R.S.).

Affiliations

  1. Department of Molecular Biophysics and Biochemistry, Yale University, PO Box 6666, New Haven, Connecticut 06511, USA

    • James B. Matthew
    • , P. C. Weber
    • , F. R. Salemme
    •  & F. M. Richards

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https://doi.org/10.1038/301169a0

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