Abstract
Extensive efforts have recently been made to characterize cells capable of mediating natural killing activity (see ref. 1 for review) and increasing evidence has arisen that these cells were heterogeneous2,3. By using the methods we have recently developed for cloning natural killer (NK) cells derived from peripheral blood4, we have analysed the heterogeneity of human NK cells. Seven cell lines showing NK activity were established and studied for 4 months. Their phenotype was determined with a series of monoclonal antibodies; anti-T1, -T3, -T4, -T8, -T11, -T12, Mo1 and each cell line appeared to have a unique phenotype. Moreover, whereas some of these lines could only kill K562 cells, the standard assay of NK activity, others displayed a broad but distinct spectrum of reactivity against a variety of human tumour and viral transformed cell lines. Taken together, these results demonstrate the phenotypic and functional heterogeneity of NK effector cells which has recently been suggested in both human and marine systems2,3,5.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Herberman, R. B. & Ortaldo, J. R. Science 214, 24 (1981).
Lust, J. A., Kumar, V., Burton, R. C., Bartlett, S. P. & Bennet, M. J. exp. Med. 154, 306 (1981).
Minato, N., Reo, L. & Bloom, B. R. J. exp. Med. 154, 750 (1981).
Hercend, T., Meuer, S., Reinherz, E. L., Schlossman, S. F. & Ritz, J. J. Immun. 129, 1299 (1982).
Zarling, J. M., Clouse, K. A., Biddison, W. E. & Kung, P. C. J. Immun. 127, 2575 (1981).
Ozer, A., Strelkauskas, A. J., Gallery, R. T. & Schlossman, S. F. Eur. J. Immun. 9, 112 (1979).
Seeley, J. K., Masucci, G., Poros, A., Klein, E. & Golub, S. H. J. Immun. 123, 1303 (1979).
Rimm, I. J., Schlossman, S. F., & Reinherz, E. L. Clin. Immun. Immunopath. 21, 134 (1981).
Dennert, G. Nature 287, 47 (1980).
Nabel, G., Bucalo, L. R., Allard, J., Wigzell, H. & Cantor, H. J. exp. Med. 153, 1582 (1981).
Reinherz, E. L. & Schlossman, S. F. in The Potential Role of T Subpopulations in Cancer Therapy (ed. Fefer, A.) (Raven, New York, in the press).
Reinherz, E. L., Kung, P. C., Goldstein, G. & Schlossman, S. F. Proc. natn. Acad. Sci. U.S.A. 78, 4061 (1979).
Abo, T. & Balch, C. M. J. Immun. 127, 1024 (1982).
Meuer, S. C., Schlossman, S. F. & Reinherz, E. L. Proc. natn. Acad. Sci. U.S.A. 79, 4395 (1982).
Reinherz, E. L., Kung, P. C., Goldstein, G. & Schlossman, S. F. J. Immun. 123, 1312 (1979).
Reinherz, E. L., Kung, P. C., Goldstein, G. & Schlossman, S. F. J. Immun. 124, 1301 (1980).
Todd, R. F., Nadler, L. M. & Schlossman, S. F. J. Immun. 126, 1435 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hercend, T., Reinherz, E., Meuer, S. et al. Phenotypic and functional heterogeneity of human cloned natural killer cell lines. Nature 301, 158–160 (1983). https://doi.org/10.1038/301158a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/301158a0
This article is cited by
-
Molecular analysis of protein interactions mediating the function of the cell surface protein CD8
Immunologic Research (1999)
-
CR3 (CD11b/CD18) expressed by cytotoxic T cells and natural killer cells is upregulated in a manner similar to neutrophil CR3 following stimulation with various activating agents
Journal of Clinical Immunology (1993)
-
Recognition of the Qa-2k tumor antigen by T cell receptor γ/δ of an immunopotentiator-induced tumoricidal T cell of mice
Cancer Immunology Immunotherapy (1993)
-
Natural killer cells in patients with pulmonary tuberculosis
Bulletin of Experimental Biology and Medicine (1991)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.