Article | Published:

Unusual splice sites revealed by mutagenic inactivation of an authentic splice site of the rabbit β-globin gene

Nature volume 301, pages 3843 (06 January 1983) | Download Citation

Subjects

Abstract

Only one of six point mutations of the sequence around one end of the larger of the introns of the rabbit β-globin gene seriously affects the normal removal of the intron and splicing of the gene. That mutation converts a GT sequence, invariably found at the 5′ end of introns, into an AT, which is no longer recognized as a signal for intron removal Instead, three normally unused (cryptic) sites are used, leading to aberrant gene transcripts. One of the cryptic sites is an exception to the invariable GT sequence.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    , , , & Proc. natn. Acad. Sci. U.S.A. 75, 4853–4857 (1978).

  2. 2.

    Nucleic Acids Res. 10, 459–471 (1982).

  3. 3.

    , & Nucleic Acids Res. 6, 3387–3398 (1979).

  4. 4.

    & Proc. natn. Acad. Sci. U.S.A. 77, 1877–1879 (1980).

  5. 5.

    , , , & Nature 283, 220–224 (1980).

  6. 6.

    Cell 23, 643–646 (1981).

  7. 7.

    , , & ICN-UCLA Symp. molec. cell. Biol. (in the press).

  8. 8.

    , , & Cell 29, 903–911 (1982).

  9. 9.

    , & Cell 29, 895–902 (1982).

  10. 10.

    , , & Science 206, 337–344 (1979).

  11. 11.

    , , & Proc. natn. Acad. Sci. U.S.A. 78, 1411–1415 (1981).

  12. 12.

    , & Gene 9, 287–305 (1980).

  13. 13.

    , , & Proc. natn. Acad. Sci. U.S.A. 72, 367–371 (1975).

  14. 14.

    , , & J. molec. Biol. 124, 343–358 (1978).

  15. 15.

    et al. ICN-UCLA Symp. molec. cell. Biol., 23, 367–385 (1981).

  16. 16.

    , , , & Molec. gen. Genet. 177, 231–242 (1980).

  17. 17.

    & Nature 293, 79–81 (1981).

  18. 18.

    , & Cell 27, 299–308 (1981).

  19. 19.

    & Proc. natn. Acad. Sci. U.S.A. 78, 133–137 (1981).

  20. 20.

    et al. Cell 28, 585–593 (1982).

  21. 21.

    et al. Proc. natn. Acad. Sci. U.S.A. 74, 4406–4410 (1977).

  22. 22.

    & Virology 52, 456–467 (1973).

  23. 23.

    , , & Cell 14, 725–731 (1978).

  24. 24.

    , & J. biol. Chem. 253, 2483–2495 (1978).

  25. 25.

    & Nature 294, 572–574 (1981).

  26. 26.

    Proc. natn. Acad. Sci. U.S.A. 79, 3493–3497 (1982).

  27. 27.

    et al. Cold Spring Harb. Meeting on RNA Processing, Abstr. 132 (1982).

  28. 28.

    , , & Nature 295, 380–384 (1982).

  29. 29.

    , , & Cell 18, 85–92 (1979).

  30. 30.

    & Proc. natn. Acad. Sci. U.S.A. 76, 71–75 (1979).

  31. 31.

    , & Nature 286, 776–779 (1980).

  32. 32.

    & Nature 286, 779–783 (1980).

  33. 33.

    et al. Nature 296, 627–631 (1982).

  34. 34.

    , , & Proc. natn. Acad. Sci. U.S.A. 78, 4471–4474 (1981).

  35. 35.

    J. molec. Biol. 50, 689–702 (1970).

  36. 36.

    , , & Gene 17, 55–63 (1982).

  37. 37.

    & Nature 289, 378–382 (1981).

  38. 38.

    & Nature 286, 634–637 (1980).

  39. 39.

    et al. Nature 275, 37–44 (1978).

  40. 40.

    & Meth. Enzym. 65, 39–43 (1980).

  41. 41.

    in DNA Tumor Viruses (ed. Tooze, J.) (Cold Spring Harbor Laboratory, New York, 1980).

  42. 42.

    , & Nucleic Acids Res. 9, 489–501 (1981).

  43. 43.

    & Meth. Enzym. 65, 499–560 (1980).

  44. 44.

    , , & Somatic Cell Genet. 6, 333–348 (1980).

  45. 45.

    & Eur. J. Biochem. 107, 303–314 (1980).

  46. 46.

    & Cell 12, 721–732 (1977).

  47. 47.

    & NucleicAcidsRes. 7, 1175–1193 (1979).

  48. 48.

    , & Nature 287, 401–408 (1980).

  49. 49.

    & Gene 10, 63–67 (1980).

  50. 50.

    Analyt. Biochem. 98, 305–309 (1979).

  51. 51.

    & Nucleic Acids Res. 8, 2255–2274 (1980).

Download references

Author information

Author notes

    • François Meyer

    Present address: Ciba-Geigy AG, CH-4000 Basel, Switzerland.

Affiliations

  1. Institut für Molekularbiologie I, Universität Zürich, 8093 Zürich, Switzerland

    • Berend Wieringa
    • , François Meyer
    • , Jakob Reiser
    •  & Charles Weissmann

Authors

  1. Search for Berend Wieringa in:

  2. Search for François Meyer in:

  3. Search for Jakob Reiser in:

  4. Search for Charles Weissmann in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/301038a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.