Abstract
The mouse major transplantation antigens H–2K, H–2D and H–2L are highly polymorphic cell-surface glycoproteins which may serve as recognition elements in cell–cell interactions1. Each antigen possesses a number of alloantigenic determinants defined by antisera of various specificities. Recently, monoclonal antibodies have been produced which redefine and extend our knowledge of these determinants2,3, but structural information has not yet been correlated with the serological definition of the antigens. We have previously reported the molecular cloning of genes for H–2Ld and H–2Dd transplantation antigens from the BALB/c mouse and the expression of these genes in mouse L cells4,5. To localize the serological determinants to discrete regions of the H–2 protein, we have now constructed new H–2 antigen genes by joining together fragments of the H–2Ld and H–2Dd genes. In L cells, these genes direct the synthesis of hybrid H–2 proteins and by using monoclonal antibodies of defined specificities, we have mapped classically defined serological specificities to structurally defined domains of the transplantation antigen protein. We conclude that polymorphic determinants recognized by monoclonal antibodies are located in functionally distinct portions of the protein.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Klein, J. Science 203, 516–521 (1979).
Ozato, K., Hansen, T. H. & Sachs, D. H. J. Immun. 125, 2473–2477 (1980).
Ozato, K. & Sachs, D. H. J. Immun. 126, 317–321 (1981).
Evans, G. A., Margulies, D. H., Ozato, K., Camerini-Otero, R. D. & Seidman, J. G. Proc. natn. acad. Sci. U.S.A. 79, 1994–1998 (1982).
Margulies, D. H. et al. J. Immun. (in the press).
Moore, K. W., Sher, B. T., Sun, Y. H., Eakle, K. A. & Hood, L. Science 215, 679–682 (1982).
Wigler, M. et al. Proc. natn. Acad. Sci. U.S.A. 76, 1373–1376 (1979).
Camerini–Otero, R. D. & Zasloff, M. A. Proc. natn. Acad. Sci. U.S.A. 77, 5079–5083 (1980).
Ozato, K., Mayer, N. M. & Sachs, D. H. Transplantation 34, 113–120 (1982).
Coligan, J. E., Kindt, T. J., Uehara, H., Martinko, J. & Nathenson, S. G. Nature 291, 35–39 (1981).
Maloy, W. L. & Coligan, J. E. Immunogenetics 16, 11–22 (1982).
Hansen, T. H. & Sachs, D. H. J. Immun. 121, 1469–1475 (1978).
Hansen, T. H., Ivanyi, P., Levy, R. B. & Sachs, D. H. Transplantation 28, 339–342 (1979).
Orn, A. et al. Nature 297, 415–417 (1982).
Weyland, C., Goronszy, J. & Hammerling, G. J. J. exp. Med. 54, 1717–1731 (1981).
Enquist, L. W., Van de Woude, G. F., Wagner, M., Smiley, J. R. & Summers, W. C. Gene 7, 335–348 (1979).
Dagert, M. & Erlich, S. D. Gene 6, 23–28 (1979).
Holmes, D. S. & Quigley, M. Analyt. Biochem. 114, 193–197 (1981).
Soberon, X., Covarrubias, L. & Bolivar, F. Gene 9, 287–305 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Evans, G., Margulies, D., Shykind, B. et al. Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens. Nature 300, 755–757 (1982). https://doi.org/10.1038/300755a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/300755a0
This article is cited by
-
Enhancement of bacteriolysis of Shuffled phage PhiX174 gene E
Virology Journal (2011)
-
Specificity of amyloid precursor-like protein 2 interactions with MHC class I molecules
Immunogenetics (2008)
-
Inactivation of Notch1 in immature thymocytes does not perturb CD4 or CD8 T cell development
Nature Immunology (2001)
-
MHC class I alloantigen specificity of Ly-49+ IL-2-activated natural killer cells
Nature (1992)
-
Isolation and properties of a Lyt-2.1-negative mutant of a Lyt-2.1/Lyt-2.2 CTL line
Immunogenetics (1991)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.