Abstract
The expression in normal rat cells of modified polyoma virus genomes, separately encoding large T, middle T or small T antigens, has allowed the investigation of the roles of these proteins in oncogenic transformation. Middle T is sufficient to transform cells of established lines but the transformants are serum dependent. Large T lacks intrinsic oncogenic potential but can relieve the serum dependence of normal and transformed cells. Middle T alone cannot transform primary rat embryo fibroblasts.
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References
Ito, Y., Spurr, N. & Dulbecco, R. Proc. natn. Acad. Sci. U.S.A. 74, 1259–1263 (1977).
Huntchinson, M. A., Hunter, T. & Eckhart, W. Cell 15, 65–80 (1978).
Hunter, T., Huntchinson, M. A. & Eckhart, W. Proc. natn. Acad. Sci. U.S.A. 75, 5917–5920 (1978).
Schaffhausen, B. S., Silver, J. E. & Benjamin, T. Proc. natn. Acad. Sci. U.S.A. 75, 79–83 (1978).
Ito, Y. in Viral Oncology (ed. Klein, G.) 447–473 (Raven, New York, 1980).
Türler, H. Molec. cell. Biochem. 32, 63–93 (1980).
Hand, R. Biochim. biophys. Acta 651, 1–24 (1981).
Triesman, R., Novak, U., Favaloro, J. & Kamen, R. Nature 292, 595–600 (1981).
Tyndall, C., La Mantia, G., Thacker, C. M., Favaloro, J. & Kamen, R. Nucleic Acids Res. 9, 6231–6250 (1981).
Rassoulzadegan, M. et al. in 35th M.D. Anderson Hospital and Tumor Inst. Symp. Fundamental Cancer Research (Raven, New York, in the press).
Treisman, R. H., Cowie, A., Favaloro, J. M., Jat, P. & Kamen, R. J. molec. appl. Genet. 1, 83–92 (1981).
Israel, M. A., Simmons, D. T., Hourihan, S. L., Rowe, W. P. & Martin, M. A. Proc. natn. Acad. Sci. U.S.A. 76, 3713–3716 (1979).
Hassell, J. A., Topp, W. C., Rifkin, D. B. & Moreau, P. E. Proc. natn. Acad. Sci. U.S.A. 77, 3978–3982 (1980).
Ito, Y. & Spurr, N. Cold Spring Harb. Symp. quant. Biol. 44, 149–147 (1980).
Ito, Y., Spurr, N. & Griffin, B. E. J. Virol. 35, 219–227 (1980).
Lania, L. et al. Virology 101, 217–227 (1980).
Novack, U., Dilworth, S. M. & Griffin, B. E. Proc. natn. Acad. Sci. U.S.A. 77, 3278–3282 (1980).
Templeton, D. & Eckhart, W. J. Virol. 41, 1014–1024 (1981).
Silver, J., Schaffhausen, B. & Benjamin, T. Cell 15, 485–496 (1978).
Staneloni, R., Fluck, M. & Benjamin, T. Virology 77, 598–609 (1977).
Rassoulzadegan, M., Gaudray, P., Canning, M., Trejo-Avila, L. & Cuzin, F. Virology 114, 489–500 (1981).
Todaro, G. J. & Green, H. J. Cell Biol. 17, 299–310 (1963).
Seif, R. & Cuzin, F. J. Virol. 24, 721–728 (1977).
Rassoulzadegan, M., Binétruy, B. & Cuzin, F. Nature 295, 257–259 (1982).
Benjamin, T. L. Proc. natn. Acad. Sci. U.S.A. 73, 1097–1100 (1970).
Hattori, J., Carmichael, G. G. & Benjamin, T. L. Cell 16, 505–513 (1979).
Lania, L., Griffiths, M., Cooke, B., Ito, Y. & Fried, M. Cell 18, 793–802 (1979).
Kaplan, P. & Ozanne, B. Virology (in the press).
Smith, H. S., Scher, C. D. & Todaro, G. J. Virology 44, 359–370 (1971).
Vogt, M. & Dulbecco, R. Proc. natn. Acad. Sci. U.S.A. 49, 171–179 (1963).
Rassoulzadegan, M. et al. Cold Spring Harb. Symp. quant. Biol. 44, 333–342 (1980).
Della Valle, G., Fenton, R. G. & Basilico, F. Cell 23, 347–355 (1981).
Martin, R. G. et al. Cold Spring Harb. Symp. quant. Biol. 44, 311–324 (1980).
Lehman, I. R. & Uyemura, D. G. Science 193, 963–969 (1976).
Griffin, B. E. & Maddock, C. J. Virol. 31, 645–656 (1979).
Medina, D. in Cancer, a Comprehensive Treatise Vol. 3 (ed. Becker, F. F.) 99–120 (Plenum, New York, 1975).
Soeda, E., Arrand, J. R., Smolar, N., Walsh, J. E. & Griffin, B. E. Nature 283, 445–453 (1980).
Pollack, R., Risser, R., Conlon, S. & Rifkin, D. Proc. natn. Acad. Sci. U.S.A. 71, 4792–4796 (1974).
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Rassoulzadegan, M., Cowie, A., Carr, A. et al. The roles of individual polyoma virus early proteins in oncogenic transformation. Nature 300, 713–718 (1982). https://doi.org/10.1038/300713a0
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DOI: https://doi.org/10.1038/300713a0
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