Oncogenes in solid human tumours

Abstract

The identification and isolation of oncogenes capable of inducing malignant transformation on transfection into rodent cells from human tumour cells has opened the possibility of deciphering the processes involved in human carcinogenesis. With the exception of three human lymphomas1, the human transforming genes so far identified have been detected in established tumour cell lines1–8, raising the possibility that they might have been activated during in vitro manipulation of the cells. However, we report here that unmanipulated human solid tumours, including carcinomas of the colon (two), lung and pancreas and an embryonal rhabdomyosarcoma, also contain dominant transforming genes. The carcinomas of the lung and pancreas and the rhabdomyosarcoma possessed a common oncogene which, like that isolated from human LX-1 lung carcinoma cells9, shares sequences with the onc gene of the Kirsten strain of murine sarcoma virus (MSV). We also show that several other human tumour cell lines, including those established from carcinomas of the colon (A2233), lung (A427 and A2182), gall bladder (A1604) and urinary bladder (A1698), possess the same oncogene. Thus a variety of human tumours, regardless of their clinical manifestations, contain a common transforming gene.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Lane, M. A., Sainten, A. & Cooper, G. M. Cell 28, 873–880 (1982).

    CAS  Article  Google Scholar 

  2. 2

    Shih, C., Padhy, L. C., Murray, M. & Weinberg, R. A. Nature 290, 261–264 (1981).

    ADS  CAS  Article  Google Scholar 

  3. 3

    Krontiris, T. G. & Cooper, G. M. Proc. natn. Acad. Sci. U.S.A. 78, 1181–1184 (1981).

    ADS  CAS  Article  Google Scholar 

  4. 4

    Lane, M. A., Sainten, A. & Cooper, G. M. Proc. natn. Acad. Sci. U.S.A. 78, 5185–5189 (1981).

    ADS  CAS  Article  Google Scholar 

  5. 5

    Murray, M. J. et al. Cell 25, 355–361 (1981).

    CAS  Article  Google Scholar 

  6. 6

    Perucho, M. et al. Cell 27, 467–476 (1981).

    CAS  Article  Google Scholar 

  7. 7

    Smith, B. L., Anisowicz, A., Chodosh, L. A. & Sager, R. Proc. natn. Acad. Sci. U.S.A. 79, 1964–1968 (1982).

    ADS  CAS  Article  Google Scholar 

  8. 8

    Pulciani, S. et al. Proc. natn. Acad. Sci. U.S.A. 79, 2845–2849 (1982).

    ADS  CAS  Article  Google Scholar 

  9. 9

    Der, C. J., Krontiris, T. G. & Cooper, G. M. Proc. natn. Acad. Sci. U.S.A. 79, 3637–3640 (1982).

    ADS  CAS  Article  Google Scholar 

  10. 10

    Graham, F. L. & van der Erb, A. J. Virology 52, 456–467 (1973).

    CAS  Article  Google Scholar 

  11. 11

    Wigler, M. et al. Cell 11, 223–232 (1977).

    CAS  Article  Google Scholar 

  12. 12

    Goldfarb, M., Shimizu, K., Perucho, M. & Wigler, M. Nature 296, 404–409 (1982).

    ADS  CAS  Article  Google Scholar 

  13. 13

    Shih, C. & Weinberg, R. A. Cell 29, 161–169 (1982).

    CAS  Article  Google Scholar 

  14. 14

    Parada, L. F., Tabin, C. J., Shih, C. & Weinberg, R. A. Nature 297, 474–478 (1982).

    ADS  CAS  Article  Google Scholar 

  15. 15

    Santos, E., Tronick, S. R., Aaronson, S. A., Pulciani, S. & Barbacid, M. Nature 298, 242–247 (1982).

    Article  Google Scholar 

  16. 16

    Ellis, R. W. et al. Nature 292, 506–511 (1981).

    ADS  CAS  Article  Google Scholar 

  17. 17

    Andersen, P. R. et al. Cell 26, 129–134 (1981).

    CAS  Article  Google Scholar 

  18. 18

    Shih, T. Y., Weeks, M. O., Young, H. A. & Scolnick, E. M. Virology 96, 64–79 (1979).

    CAS  Article  Google Scholar 

  19. 19

    Shilo, B. Z. & Weinberg, R. A. Nature 289, 607–609 (1981).

    ADS  CAS  Article  Google Scholar 

  20. 20

    Peto, R. in Banbury Report 9: Quantification of Occupational Cancer (eds Peto, R. & Schneiderman, M.) 269–284 (Cold Spring Harbor Laboratory, New York, 1981).

    Google Scholar 

  21. 21

    Bubenik, J. et al. Int. J. Cancer 11, 765–773 (1973).

    CAS  Article  Google Scholar 

  22. 22

    Giard, D. J. et al. J. natn. Cancer Inst. 51, 1417–1423 (1973).

    CAS  Article  Google Scholar 

  23. 23

    Rasheed, S., Nelson-Rees, W. A., Toth, E. M., Arnstein, P. & Gardner, M. B. Cancer d33, 1027–1033 (1974).

    CAS  Article  Google Scholar 

  24. 24

    Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    CAS  Article  Google Scholar 

  25. 25

    Tsuchida, N., Ryder, T. & Ohtsubo, E. Science 217, 937–939 (1982).

    ADS  CAS  Article  Google Scholar 

  26. 26

    Barbacid, M., Lauver, A. L. & Devare, S. J. J. Virol. 33, 196–207 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  27. 27

    Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83–88 (1974).

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Pulciani, S., Santos, E., Lauver, A. et al. Oncogenes in solid human tumours. Nature 300, 539–542 (1982). https://doi.org/10.1038/300539a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.