Abstract
Garson and Coakham demonstrated a monoclonal antibody (UCHT1) which specifically labels Purkinje cells in the cerebellar cortex1. To investigate mouse mutants with cerebellar lesions, we obtained some of the purified antibody from Beverley2, who derived it as an antibody to a human T-cell lymphocyte surface antigen. We have used the indirect immunoperoxidase method to label with the antibody Purkinje cells in mutant and normal mice, frog, toad, goldfish, dogfish and 3-acetylpyridine-treated rat. We report here that in the mutant mice tested, only the Purkinje cells in staggerer did not stain. The Purkinje cells of frog, toad and goldfish were also unstained but those in the dogfish were labelled. The Purkinje cells in rats which had been injected with 3-acetylpyridine to destroy the climbing fibres, were found to stain with the antibody. These results suggest phylogenetic as well as ontogenetic differences in antigenicity of Purkinje cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Garson, J. A., Beverley, P. C. L., Coakham, H. B. & Harper, E. I. Nature 298, 375–377 (1982).
Beverley, P. C. L. & Callard, R. E. Eur. J. Immun. 11, 329–334 (1981).
Caddy, K. W. T. & Sidman, R. L. Devl Brain Res. 1, 221–236 (1981).
Caddy, K. W. T. & Biscoe, T. J. Phil. Trans. R. Soc. B287, 167–201 (1979).
Sotelo, C. Brain Res. 94, 19–44 (1975).
Hamburgh, M. Devl Biol. 8, 165–185 (1963).
Herrup, K. & Mullen, R. J. Brain Res. 172, 1–12 (1979).
Bradley, P. & Berry, M. Brain Res. 142, 135–141 (1978).
Mariani, J., Crepel, F., Mikoshiba, K., Changeaux, J. P. & Sotelo, C. Phil. Tram. R. Soc. B281, 1–28 (1977).
Crepel, F. & Mariani, J. Brain Res. 98, 135–147 (1975).
Crepel, F., Delhaye-Bouchard, N., Guastavino, J. M. & Sampaio, I. Nature 283, 483–484 (1980).
Llinas, R., Walton, K., Hillman, D. E. & Sotelo, C. Science 190, 1230–1231 (1975).
Armstrong, D. M. Physiol. Rev. 54, 358–417 (1974).
Hirano, A. & Dembitzer, H. M. J. Neuropath. exp. Neurol. 34, 1–11 (1975).
Landis, D. M. D. & Sidman, R. L. J. comp. Neurol. 179, 831–863 (1978).
Herrup, K. & Mullen, R. J. Brain Res. 178, 443–457 (1979).
Llinas, R. & Hillman, D. E. Neurobiology of Cerebellar Evolution and Development (ed. Llinas, R.) 43–73 (American Medical Association, 1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Caddy, K., Patterson, D. & Biscoe, T. Use of the UCHT1 monoclonal antibody to explore mouse mutants and development. Nature 300, 441–443 (1982). https://doi.org/10.1038/300441a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/300441a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.