The repair of UV-damaged DNA in Escherichia coli is controlled by the uvr genes (A, B and C)1,2. These genes are also thought to be involved in the repair of chemically damaged DNA3–5, however their specific roles in repairing the multiplicity of different adducts that can be produced by chemical carcinogens are not well understood. We have investigated the repair of DNA damaged by different chemical carcinogens and transfected into E. coli strains with mutations in different uvr genes. The major product of DNA damage by N-acetoxy-2-acetylaminofluorene (NA-AAF) is N-(guanin-C8-yl)-2-acetylaminofluorene (G-C8-AAF)6. Our results suggest that, like UV-induced pyrimidine dimers, the repair of this adduct requires all three uvr genes. However, the repair of DNA damaged by N-hydroxy-aminofluorene (N-OH-AF, the major adduct of which is N-(guanin-C8-yl)-2-aminofluorene, G-C8-AF) seems only to require the uvrC gene.
Subscribe to Journal
Get full journal access for 1 year
only $3.90 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Howard-Flanders, P., Boyce, R. P. & Theriot, L. Genetics 53, 1119–1136 (1966).
Seeberg, E. Prog. Nucleic Acid Res. molec. Biol. 26, 217–226 (1981).
Venitt, S. & Tarmy, E. M. Biochim. biophys. Acta 287, 38–51 (1972).
Ikenaga, M., Ichikawa-Ryo, H. & Kondo, S. J. molec. Biol. 92, 341–356 (1975).
Hanawalt, P. C., Cooper, P. K., Ganesan, A. K. & Smith, C. A. A. Rev. Biochem. 48, 783–836 (1979).
Miller, E. C. & Miller, J. A. Cancer 47, 2327–2345 (1981).
Moore, P., Bose, K. K., Rabkin, S. D. & Strauss, B. S. Proc. natn. Acad. Sci. U.S.A. 78, 110–114 (1981).
Yamamoto, Y., Katsuki, M., Sekiguchi, M. & Otsuji, N. J. Bact. 135, 144–152 (1978).
Trifonov, E. N., Shafranovskaya, V. N., Frank-Kamenetskii, M. D. & Lazurkin, Yu. S. Molec. Biol. 2, 698–705 (1968).
Fuchs, R. P. P. & Daune, M. P. Biochemistry 13, 4435–4440 (1974).
Leng, M., Ptak, M. & Rio, P. Biochem. biophys. Res. Commun. 96, 1095–1102 (1980).
Visser, A. & Westra, J. G. Carcinogenesis 2, 737–740 (1981).
Howard, P. C., Casciano, D. A., Beland, F. A. & Shaddock, J. G. Jr Carcinogenesis 2, 97–102 (1981).
Ames, B. N., Gurney, E. G., Miller, J. A. & Bartsch, H. Proc. natn. Acad. Sci. U.S.A. 69, 3128–3132 (1972).
Barnes, W. M. J. molec. Biol. 119, 83–99 (1978).
Howard-Flanders, P. A. Rev. Biochem. 37, 175–200 (1968).
Westra, J. G. & Visser, A. Cancer Lett. 8, 155–162 (1979).
About this article
Proceedings of the National Academy of Sciences (2018)
Differential survival of Escherichia coli uvrA, uvrB, and uvrC mutants to psoralen plus UV-A (PUVA): Evidence for uncoupled action of nucleotide excision repair to process DNA adducts
Journal of Photochemistry and Photobiology B: Biology (2010)
Proceedings of the National Academy of Sciences (2010)
Malondialdehyde, a major endogenous lipid peroxidation product, sensitizes human cells to UV- and BPDE-induced killing and mutagenesis through inhibition of nucleotide excision repair
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis (2006)
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis (2005)