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Specific protein-nucleic acid recognition in ribonuclease T1–2′-guanylic acid complex: an X-ray study

Nature volume 299, pages 27–31 (1982)Cite this article

Abstract

RNase T1 is folded into an α-helix of 4.5 turns, covered by a four-strand antiparallel β-sheet. Specific recognition of 2′-guanylic acid arises from hydrogen bonding between main chain peptide groups and the O-6 and N-1–H of guanine, as well as from stacking of Tyr 45 on guanine. At the active site, Glu 58, His 92 and Arg 77 are involved in phosphodiester hydrolysis.

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Author information

Authors and Affiliations

  1. Abteilung Chemie, Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Strasse 3, D-3400, Göttingen, FRG

    Udo Heinemann

  2. Institut für Kristallographie, Freie Universität Berlin, Takustrasse 6, D-1000, Berlin, 33, FRG

    Wolfram Saenger

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  1. Udo Heinemann
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  2. Wolfram Saenger
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Heinemann, U., Saenger, W. Specific protein-nucleic acid recognition in ribonuclease T1–2′-guanylic acid complex: an X-ray study. Nature 299, 27–31 (1982). https://doi.org/10.1038/299027a0

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