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Amplification of endogenous myc-related DNA sequences in a human myeloid leukaemia cell line

Nature volume 298, pages 679–681 (1982)Cite this article

Abstract

Malignant transformation of cells by acute transforming RNA tumour viruses is mediated by the expression of certain specific pro viral DNA sequences (‘oncogenes’). These sequences have been well characterized and, in many cases, molecularly cloned1–8. These viral oncogenes are related to similar genes found in normal uninfected cells9,10. Moreover, these particular sequences are highly conserved in evolution4,11, suggesting that these genes have an important, albeit unknown, role in normal cell function. It has been suggested that an increased dosage of products of such endogenous oncogenes may be responsible for malignant transformation10,12,13. For example, increased expression of the endogenous chick c-myc oncogene has been observed in avian leukosis virus-induced transformation of chick bursal lymphocytes12. Here we demonstrate that sequences in normal human DNA homologous to the avian myc oncogene are present in multiple copies in the chromosomal DNA of the human leukaemia cell line HL-60. Other transformation-specific genes derived from the Abelson leukaemia virus4 and feline sarcoma virus6 are not amplified in HL-60. This myc-related gene amplification is not present in other cultured human myeloid leukaemia cells, including K-56214 and KG-115.

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Authors and Affiliations

  1. Veterans Administration Hospital, 4435 Beacon Avenue South, Seattle, Washington, 98108, USA

    Steven Collins

  2. Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, Washington, 98104

    Mark Groudine

  3. Department of Radiation Oncology, University of Washington Hospital, Seattle, Washington, 98195, USA

    Mark Groudine

Authors
  1. Steven Collins
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  2. Mark Groudine
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Collins, S., Groudine, M. Amplification of endogenous myc-related DNA sequences in a human myeloid leukaemia cell line. Nature 298, 679–681 (1982). https://doi.org/10.1038/298679a0

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