Abstract
Hypogonadism in the mutant hpg mouse is characterized by a deficiency of hypothalamic gonadotropin releasing hormone (GnRH)1. Affected male mice exhibit immature reproductive organs, small abdominal testes and low pituitary and plasma gonadotropin concentrations. Recent studies have demonstrated the potential of fetal brain transplants to establish functional connections with host tissues2. We therefore sought to use this approach to correct the hpg deficit. Fetal preoptic area (POA) (a site of GnRH production) from unaffected animals of the hpg strain was transplanted into the anterior third ventricle of adult hpg mice. We report that in such implanted animals, killed 2 months post-implantation, the POA grafts contained GnRH neurones, from which GnRH-positive fibres could be traced to capillaries of the median eminence. Hypothalamic GnRH and pituitary and plasma gonadotropin concentrations were increased compared with levels in untreated (hpg) animals. The testes were enlarged and had descended into the scrotum. Evidence of full spermatogenesis and interstitial cell development was present in testicular sections. No such effects were seen with transplants of cortical tissue.
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References
Cattanach, B. M., Iddon, C. A., Charlton, H. M., Chiappa, S. A. & Fink, G. Nature 269, 338–340 (1977).
Bjorklund, A., Stenevi, U. & Svendgaard, N.-A. Nature 262, 787–790 (1976).
Schwanzel-Fukuda, M. & Silverman, A. J. J. comp. Neurol. 191, 213–225 (1980).
Gross, D. S. & Baker, B. L. Am. J. Anat. 148, 195–216 (1977).
Iddon, C. A., Charlton, H. M. & Fink, G. J. Endocr. 85, 105–110 (1980).
Bamber, S., Iddon, C. A., Charlton, H. M. & Ward, B. J. J. Reprod. Fert. 58, 249–252 (1980).
Moss, R. L., Riskand, P. & Dudley, C. in Central Nervous System Effects of Hypothalamic Hormones and Other Peptides (eds Collu, R., Barbeau, A., Ducharme, J. R. & Rochefort, J.-G.), 345 (Raven, New York, 1979).
Lund, R. D. & Hauschka, S. D. Science 193, 582–584 (1976).
Hallas, B. H., Oblinger, M. M. & Das, G. D. Brain Res. 196, 242–246 (1980).
Kromer, L. F., Bjorklund, A. & Stenevi, U. Brain Res. 210, 153–171 (1981).
Azmitia, E. C., Perlow, M. J., Brennan, M. J. & Lauder, J. M. Brain Res. Bull. 7, 703–710 (1981).
Stenevi, U. et al. Cell Tissue Res. 205, 217–228 (1980).
Perlow, M. J. et al. Science 204, 643–647 (1979).
Björklund, A., Donnett, S. B., Stenevi, U., Lewis, M. F. & Iversen, S. D. Brain Res. 199, 307–333 (1980).
Gash, D. & Scott, D. E. Cell Tissue Res. 211, 191–206 (1980).
Gash, D., Sladek, J. R. Jr & Sladek, C. Science 210, 1367–1369 (1981).
Kirk, R. F. Experimental Design: Procedures for the Behavioral Sciences, 69–98; 493–497 (Wadsworth, California, 1968).
Araki, S., Ferin, M., Zimmerman, E. A. & vande Wiele, R. I. Endocrinology 96, 644–650 (1975).
Hsu, S.-M., Raine, L. & Fanger, H. J. Histochem. Cytochem. 29, 577–580 (1981).
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Krieger, D., Perlow, M., Gibson, M. et al. Brain grafts reverse hypogonadism of gonadotropin releasing hormone deficiency. Nature 298, 468–471 (1982). https://doi.org/10.1038/298468a0
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DOI: https://doi.org/10.1038/298468a0
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