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Synthesis and assembly of hepatitis B virus surface antigen particles in yeast

Nature volume 298, pages 347–350 (1982)Cite this article

Abstract

The surface antigen of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.

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References

  1. Vyas, G. N., Cohen, S. N. & Schmid, R. (eds) Viral Hepatitis. A Contemporary Assessment of Etiology, Epidemiology, Pathogenesis and Prevention (Franklin Institute Press, Philadelphia, 1978).

  2. Zuckerman, A. J. J. Tox. envir. Hlth 5, 275–280 (1979).

    CAS  Article  Google Scholar 

  3. Prince, A. M. Hepatology 1, 73 (1981).

    CAS  Article  Google Scholar 

  4. Summers, J., O'Connell, A. & Millman, I. Proc. natn. Acad. Sci. U.S.A. 72, 4597–4601 (1975).

    ADS  CAS  Article  Google Scholar 

  5. Pasek, M. et al. Nature 282, 575–579 (1979).

    ADS  CAS  Article  Google Scholar 

  6. Galibert, F., Mandart, E., Fitoussi, F., Tiollais, P. & Charnay, P. Nature 281, 646–650 (1979).

    ADS  CAS  Article  Google Scholar 

  7. Valenzuela, P., Quiroga, M., Zaldivar, J., Gray, P. & Rutter, W. J. in Animal Virus Genetics (eds Fields, B. N., Jaenisch, R. & Fox, C. F.) 55–77 (Academic, New York, 1980).

    Google Scholar 

  8. Robinson, W. S. & Greenman, R. C. J. Virol. 13, 1231–1236 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Hruska, J. F. & Robinson, W. S. J. med. Virol. 1, 119–124 (1977).

    CAS  Article  Google Scholar 

  10. Shih, J. W. K. & Gerin, J. L. J. Virol. 21, 1219–1222 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Peterson, D. L. J. biol. Chem. 256, 6975–6983 (1981).

    CAS  PubMed  Google Scholar 

  12. Cabral, G. A. et al. J. gen. Virol. 38, 339–350 (1978).

    CAS  Article  Google Scholar 

  13. Valenzuela, P. et al. Nature 280, 815–819 (1979).

    ADS  CAS  Article  Google Scholar 

  14. Burrell, C. J., Mackay, P., Greenway, P. J., Hofschneider, P. H. & Murray, K. Nature 279, 43–47 (1979).

    ADS  CAS  Article  Google Scholar 

  15. Sninsky, J. J., Siddiqui, A., Robinson, W. S. & Cohen, S. N. Nature 279, 346–348 (1979).

    ADS  CAS  Article  Google Scholar 

  16. Edman, J. C., Hallewell, R. A., Valenzuela, P., Goodman, H. M. & Rutter, W. J. Nature 291, 503–506 (1981).

    ADS  CAS  Article  Google Scholar 

  17. Mackay, P. et al. Proc. natn. Acad. Sci. U.S.A. 78, 4510–4514 (1981).

    ADS  CAS  Article  Google Scholar 

  18. Novick, P., Field, C. & Schekman, R. Cell 21, 205–225 (1980).

    CAS  Article  Google Scholar 

  19. Beggs, G. D. Nature 275, 104–109 (1978).

    ADS  CAS  Article  Google Scholar 

  20. Petes, P. D. A. Rev. Biochem. 49, 845–876 (1980).

    CAS  Article  Google Scholar 

  21. Ammerer, G., Hitzeman, R. A., Hagie, F. E., Barta, A. & Hall, B. D. Recombinant DNA, Proc. 3rd Cleveland Symp. Macromolecules (ed. Walton, A. G.) 185–197 (Elsevier, Amsterdam, 1981).

    Google Scholar 

  22. Hitzeman, R. A. et al. Nature 293, 717–722 (1981).

    ADS  CAS  Article  Google Scholar 

  23. Gerin, J. L., Purcell, R. H., Hoggan, M. D., Holland, P. V. & Chanock, R. M. J. Virol. 4, 763–768 (1969).

    CAS  PubMed  PubMed Central  Google Scholar 

  24. Gerin, J. L., Holland, P. V. & Purcell, R. H. J. Virol. 7, 569–576 (1971).

    CAS  PubMed  PubMed Central  Google Scholar 

  25. Macnab, G. M., Alexander, J. J., Letcasas, G., Bey, E. M. & Urbanowicz, J. M. Br. J. Cancer 34, 509–515 (1976).

    CAS  Article  Google Scholar 

  26. Alexander, J. J., Bey, E. M., Geddes, E. W. & Letcasas, G. S. Afr. med. J. 50, 2124–2128 (1976).

    CAS  PubMed  Google Scholar 

  27. Tschumper, G. & Carbon, J. Gene 10, 157–166 (1980).

    CAS  Article  Google Scholar 

  28. Birnboim, H. C. & Doly, J. J. Nucleic Acids Res. 7, 1513–1523 (1979).

    CAS  Article  Google Scholar 

  29. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 490–565 (1980).

    Google Scholar 

  30. Martial, J. A., Hallewell, R. A., Baxter, J. D. & Goodman, H. M. Science 205, 602–607 (1979).

    ADS  CAS  Article  Google Scholar 

  31. Laemmli, U. K. Nature 227, 680–685 (1977).

    ADS  Article  Google Scholar 

  32. Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83–88 (1974).

    CAS  Article  Google Scholar 

  33. Cohen, B. J. & Richmond, J. E. Nature 296, 677–678 (1982).

    ADS  CAS  Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of California, San Francisco, California, 94143

    Pablo Valenzuela, Angelica Medina & William J. Rutter

  2. Chiron Corporation, 4560 Horton, Emeryville, California, 94608, USA

    Pablo Valenzuela

  3. Department of Genetics, SK-50, University of Washington, Seattle, Washington, 98195, USA

    Gustav Ammerer & Benjamin D. Hall

Authors
  1. Pablo Valenzuela
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  2. Angelica Medina
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  3. William J. Rutter
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  4. Gustav Ammerer
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  5. Benjamin D. Hall
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Valenzuela, P., Medina, A., Rutter, W. et al. Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature 298, 347–350 (1982). https://doi.org/10.1038/298347a0

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