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Synthesis and assembly of hepatitis B virus surface antigen particles in yeast

Abstract

The surface antigen of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.

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Valenzuela, P., Medina, A., Rutter, W. et al. Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature 298, 347–350 (1982). https://doi.org/10.1038/298347a0

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  • DOI: https://doi.org/10.1038/298347a0

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