Abstract
Colony-stimulating factors (CSF) are glycoproteins essential for the formation of colonies of mature haematopoietic cells by single immature progenitor cells (colony-forming cells (CFC)). The number of colonies in semi-solid agar cultures can be used to determine the CSF concentration in biological fluids1. Various murine cells2–6 are able to produce CSF in vitro. Supernatants of spleen cell cultures consisting of a mixture of T cells, B cells and macrophages are found to contain neutrophil-granulocyte–macrophage CSF (GM-CSF), CSF for megakaryocytes (Meg-CSF), eosinophilic granulocytes (Eo-CSF) and erythroid cells (E-CSF)5. The producer cells of these different CSF types, however, have not been defined7. We therefore determined here whether normal T cells produce CSF in vitro and whether GM-CSF, Meg-CSF, Eo-CSF and E-CSF are produced by different T-cell subsets or the progeny of single T-cell clones. Our results indicate that T cells at a high frequency (one out of three) produce CSF at variable amounts even in the absence of accessory cells following mitogen stimulation.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Metcalf, D. in Tissue Growth Factors, (ed. Baserga, R.) (Springer, New York, 1980).
Stanley, E. R. & Heard, P. M. J. biol. Chem. 252, 4305–4312 (1977).
Staber, F. G. et al. Cell Immun. 37, 174–187 (1978).
Quesenberry, P. J., Gimbrone, M. A. & McDonald, M. J. Expl Hemat. 6, Suppl. 3, 4 (1978).
Metcalf, D. & Johnson, G. R. J. cell. Physiol. 96, 31–42 (1978).
Parker, J. W. & Metcalf, D. Immunology 26, 1039–1049 (1974).
Metcalfe, D. in Biology of the Lymphokines (eds Cohen, S., Pick, E. & Oppenheim, J. J.) 515–525 (Academic, New York, 1973).
Krammer, P. H., Marcucci, F., Waller, M. & Kirchner, H. Eur. J. Immun. 12, 200–204 (1982).
Krammer, P. H. et al. in Isolation, Characterization, and Utilization of T Lymphocyte Clones (eds Fathman, G. & Fitch, F.) 253–262 (Academic, New York, 1982).
Krammer, P. H. & Michnay, A. in Mechanisms of Lymphocyte Activation, 357–360 (Elsevier, Amsterdam, 1981).
Krammer, P. H. in The Immune System, 304–310 (Karger, Basel, 1981).
Eichmann, K., Falk, I., Melchers, I. & Simon, M. M. J. exp. Med. 152, 477–492 (1980).
Staber, F. G. & Johnson, R. J. cell. Physiol. 105, 143–152 (1980).
Porter, E. H. & Berry, R. J. Br. J. Cancer 17, 583–595 (1963).
Metcalf, D., Johnson, G. R. & Burgess, A. W. Blood 55, 138–147 (1980).
Schrader, J. W., Arnold, B. & Clark-Lewis, J. Nature 283, 197–199 (1980).
Nabholz, M. et al. Transplant Rev. 51, 125–156 (1980).
Marcucci, F., Waller, M., Kirchner, H. & Krammer, P. H. Nature 291, 79–81 (1981).
Waller, M., Marcucci, F., Kirchner, H., Michnay, A. & Krammer, P. H. Immun. Lett. 3, 263–266 (1981).
Isakson, P., Puré, E., Vitetta, E. S. & Krammer, P. H. J. exp. Med. 155, 734–749 (1982).
Krammer, P. H. et al. UCLA Symp. molec. cell. Biol. 24 (in the press).
Nabel, G., Greenberger, J. S., Sakekeeny, M. A. & Cantor, H. Proc. natn. Acad. Sci. U.S.A. 78, 1157–1161 (1981).
Schreier, M. H. & Iscove, N. N. Nature 287, 228–300 (1980).
Burgess, A. W., Metcalf, D., Russell, S. H. M. & Nicola, N. A. Biochem. J. 185, 301–314 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Staber, F., Hültner, L., Marcucci, F. et al. Production of colony-stimulating factors by murine T cells in limiting dilution and long-term cultures. Nature 298, 79–82 (1982). https://doi.org/10.1038/298079a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/298079a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
