Abstract
Transfer of a 36-kilobase piece of human DNA containing the β-interferon (IFN-β) gene into mouse Ltk− cells leads to transient expression of human interferon even without an exogenous inducer. A low level of human interferon expression is also found in most stable clones containing the transferred DNA. With double-stranded RNA or Newcastle disease virus (NDV) as inducer, human interferon expression is greatly increased. The induced transcript is identical to normal human IFN-β mRNA. Neighbouring genes contained on the transferred DNA are co-induced but are not essential for the production of human interferon in mouse L cells.
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References
Stewart, W. E. II The Interferon System (Springer, Vienna, 1979).
Field, A. K., Tytell, A. A., Lampson, G. P. & Hillemann, M. R. Proc. natn. Acad. Sci. U.S.A. 58, 1004–1010 (1967).
Raj, N. B. K. & Pitha, P. M. Proc. natn. Acad. Sci. U.S.A. 77, 4918–4922 (1980).
Vilcek, J., Ng, M. H. & Friedman-Kien, A. E. J. Virol. 2, 648–650 (1968).
Raj, N. B. K. & Pitha, P. M. Proc. natn. Acad. Sci. U.S.A. 78, 7426–7430 (1981).
Vilcek, J., Havel, E. A. & Kohase, M. J. infect. Dis. 133, A22–A29 (1976).
Tomkins, G. M. et al. Cold Spring Harb. Symp. quant. Biol. 31, 349–360 (1966).
Gross, G., Mayr, U. & Collins, J. The Biology of the Interferon System (eds De Maeyer, E., Galasso, G. & Schellekens, H.) 85–90 (Elsevier, Amsterdam, 1981).
Gross, G., Bruns, W., Mayr, U. & Collins, J. FEBS Lett. 139, 201–204 (1982).
Gross, G. et al. Nucleic Acids Res. 9, 2495–2507 (1981).
Wigler, M. et al. Cell 16, 777–785 (1979).
Lindenmaier, W., Hauser, H., Wilke, J. & Schütz, G. Nucleic Acids Res. 10, 1243–1256 (1982).
Southern, E. Meth. Enzym. 68, 152–176 (1980).
Fujisawa, J., Iwakura, Y. & Kawade, Y. J. biol. Chem. 253, 8677–8679 (1978).
Graves, H. E. & Maeger, A. J. gen. Virol. 47, 489–495 (1980).
Wiranowska-Stewart, M., Chudzio, T. & Stewart, W. E. J. gen. Virol. 37, 221–223 (1977).
Hochkeppel, H. K., Menge, U. & Collins, J. Eur. J. Biochem. 118, 437–442 (1981).
Berk, A. J. & Sharp, P. A. Cell 12, 721–732 (1977).
Weaver, R. F. & Weissmann, C., Nucleic Acids Res. 7, 1175–1193 (1979).
Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).
Degrave, W., Derynck, R., Tavernier, J., Haegeman, G. & Fiers, W. Gene 14, 137–143 (1981).
Breathnach, R., Mantei, N. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 77, 740–744 (1980).
Wold, B. et al. Proc. natn. Acad. Sci. U.S.A. 76, 5684–5688 (1979).
Hynes, N. E., Kennedy, N., Rahmsdorf, U. & Groner, B. Proc. natn. Acad. Sci., U.S.A. 78, 2038–2042 (1981).
Lee, F., Mulligan, R., Berg, P. & Ringold, G. Nature 294, 228–232 (1981).
Kurtz, D. T. Nature 291, 629–631 (1981).
Capecchi, M. R. Cell 22, 479–488 (1980).
Banerji, S. R. & Schaffner, W. Cell 27, 299–308 (1981).
Scangos, G. & Ruddle, F. H. Gene 14, 1–10 (1981).
Rusconi, S. & Schaffner, W. Proc. natn. Acad. Sci. U.S.A. 78, 5051–5055 (1981).
Ohno, S. & Taneguchi, T. Nucleic Acids Res. 10, 967–977 (1982).
Graham, F. L. & Van der Eb, A. J. Virology 52, 456–467 (1973).
Lämmli, U. K. Nature 227, 680–685 (1970).
Towbin, H., Steahelin, T. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).
Mantei, N. & Weissmann, C. Nature 297, 128–132 (1982).
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Hauser, H., Gross, G., Bruns, W. et al. Inducibility of human β-interferon gene in mouse L-cell clones. Nature 297, 650–654 (1982). https://doi.org/10.1038/297650a0
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DOI: https://doi.org/10.1038/297650a0
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