Abstract
Mice primed with 4-hydroxy-3-nitrophenylacetyl-O-succini-mide (NP-O-Su) generate hapten-specific T cell-mediated contact or cutaneous hypersensitivity (CS) responses1. Analysis of the effector T cells mediating these in vivo responses suggested that multiple T-cell subpopulations were involved2. It has not been possible previously to separate these cell populations physically by conventional means. Therefore, to separate these cells and to provide a uniform source of CS effector cells for future analyses, we hybridized the CS effector cell population with AKR-derived BW5147 thymoma cells. We now describe five H–2-restricted hybridoma T-cell clones isolated from this fusion. Each hybridoma possesses haptenspecific CS activity and four also crossreact with 4-hydroxy-3-nitrophenylacetyl (NP) derivatives. Two of the hybridoma clones are specific for NP associated with H–2I gene products; two others for NP associated with H–2D products; and one for NP associated with H–2K gene products.
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Minami, M., Okuda, K., Sunday, M. et al. H–2K-, H–2I- and H–2D- restricted hybridoma contact sensitivity effector cells. Nature 297, 231–233 (1982). https://doi.org/10.1038/297231a0
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DOI: https://doi.org/10.1038/297231a0
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