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Protein dynamics investigated by the neutron diffraction–hydrogen exchange technique

Nature volume 296, pages 713721 (22 April 1982) | Download Citation

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Abstract

A new approach, using neutron diffraction and the hydrogen exchange (H/D) technique, has been used to study the extent and nature of the inherent conformational fluctuations in the protein, trypsin. The observed pattern of exchange was used to investigate systematic relationships between exchangeable sites and structural and chemical properties of the molecule. Results of this analysis indicate that hydrogen-bonding structure is the dominant factor governing rates of exchange. The model of conformational mobility which best explains the experimental findings involves a localized disruption of the secondary structure within different regions of the protein molecule, each limited in extent to the breaking of a small number of hydrogen bonds.

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References

  1. 1.

    & in The Enzymes Vol. 1, 2nd edn (eds Boyer,D., Lardy, H. & Myrback, K.) 443 (Academic, New York, 1959).

  2. 2.

    & Adv. Protein Chem. 33, 73 (1979).

  3. 3.

    , & J. molec. Biol. 130, 231 (1979).

  4. 4.

    , & Nature 280, 558 (1979).

  5. 5.

    , & Nature 267, 585 (1977).

  6. 6.

    & in Structure and Stability of Biological Macromolecules, 65 (Dekker, New York, 1969).

  7. 7.

    A. Rev. Biochem. 44, 453 (1975).

  8. 8.

    J. theor. Biol. 58, 269 (1976).

  9. 9.

    & Biochim. biophys. Acta 14, 574 (1954).

  10. 10.

    , & in A Laboratory Manual of Analytical Techniques in Protein Chemistry (eds Alexander, P.& Block, R. J.) 101 (Pergamon, New York, 1960).

  11. 11.

    & Adv. Protein Chem. 21, 287 (1966).

  12. 12.

    , & A. Rev. Biochem. 41, 903 (1972).

  13. 13.

    et al. Biophys. J. 32, 557 (1980).

  14. 14.

    & A. Rev. Biophys. Bioengng 8, 99 (1979).

  15. 15.

    & Colloques int. Cent. natn. Rech. Scient. 246, 53 (1975).

  16. 16.

    & J. molec. Biol 130, 1 (1979).

  17. 17.

    & J. molec. Biol. 134, 75 (1979).

  18. 18.

    & Biochemistry 17, 3325 (1978).

  19. 19.

    & J. molec. Biol. 105, 409 (1976).

  20. 20.

    & J. molec. Biol. 133, 399 (1979).

  21. 21.

    & Adv. Protein Chem. 12, 133 (1957).

  22. 22.

    A. Rev. Biochem. 32, 269 (1963).

  23. 23.

    in Structure and Stability of Biological Macromolecules, 291 (Dekker, New York, 1969).

  24. 24.

    J. molec. Biol. 33, 491 (1968).

  25. 25.

    Trans. Faraday, Soc. B42, 187 (1946).

  26. 26.

    , & J. Am. chem. Soc. 78, 1107 (1956).

  27. 27.

    Biochim. biophys. Acta23, 494 (1957).

  28. 28.

    & Biochemistry 7, 2431 (1968).

  29. 29.

    & Biochemistry 7, 2446 (1968).

  30. 30.

    , & Biochimie 62, 563 (1980).

  31. 31.

    & Biochemistry 20, 6462 (1981).

  32. 32.

    Brookhaven Symp. Biol. 27, VIII–24 (1975).

  33. 33.

    & J. appl. Crystallogr. 13, 563 (1980).

  34. 34.

    & Protein Data Bank (Brookhaven National Laboratory, 1977).

  35. 35.

    J. molec. Biol. 146, 143 (1981).

  36. 36.

    , & Biochemistry 11, 150 (1972).

  37. 37.

    & Biochemistry 21, 1 (1982).

  38. 38.

    & J. Am. chem. Soc. 87, 2721 (1965).

  39. 39.

    , & J. Am. chem. Soc. 89, 6024 (1967).

  40. 40.

    & Proc. natn. Acad. Sci. U.S.A. 68, 1199 (1971).

  41. 41.

    & J. Am. chem. Soc. 92, 5546 (1970).

  42. 42.

    & A. Rev. Biochem. 47, 251 (1978).

  43. 43.

    et al. Nature 28, 563 (1979).

  44. 44.

    & Biopolymers 19, 1049 (1980).

  45. 45.

    , & Biochemistry 20, 4697 (1981).

  46. 46.

    & J. molec. Biol. 68, 187 (1972).

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Affiliations

  1. Department of Biology, Brookhaven National Laboratory, Upton, New York 11973, USA

    • A. A. Kossiakoff

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https://doi.org/10.1038/296713a0

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