Letter | Published:

Tandem repeats in the N-terminal sequence of a proline-rich protein from corn endosperm

Nature volume 296, pages 678679 (15 April 1982) | Download Citation

Subjects

Abstract

Zeins, the prolamines of corn (Zea mays), are the most abundant storage protein fraction in corn endosperm. Like prolamines of other cereals, zeins are readily soluble in 60–70% alcohol solutions but are insoluble in aqueous buffer systems. The other major storage protein fraction in corn endosperm is glutelin, which is extractable with either dilute alkali or detergent. We have now extracted from corn endosperm a prolinerich, zein-like protein fraction which we have purified by chromatography on phosphocellulose. Study of the N-terminal sequences of three chromatographic fractions for homologies shows that they are essentially identical. These N-terminal sequences include a hexapeptide Pro-Pro-Pro-Val-His-Leu, which is repeated in tandem at least six times, and probably eight times or more.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    & C.r. hebd. Séanc. Acad. Sci., Paris 266 D, 2302–2305 (1968).

  2. 2.

    , & J. agric. Fd Chem. 17, 1301–1305 (1969).

  3. 3.

    & Bull. Soc. Chim. Biol. 52, 1021–1037 (1970).

  4. 4.

    , & High Quality Protein Maize, 291 (Dowden, Hutchinson & Ross, Stroudsburgh, Pennsylvania, 1975).

  5. 5.

    & J. agric. Fd Chem. 19, 1144–1150 (1971).

  6. 6.

    & Biochim. biophys. Acta 251, 57–59 (1971).

  7. 7.

    & Cereal Chem. 54, 1223–1228 (1977).

  8. 8.

    , , & Cereal Chem. 58, 534–537 (1981).

  9. 9.

    , & Cereal Chem. 58, 275–281 (1981).

  10. 10.

    Biochim. biophys. Acta 359, 142–151 (1974).

  11. 11.

    , , , & J. exp. Bot. 31, 393–408 (1980).

  12. 12.

    , & J. Biochem. 87, 1071–1077 (1980).

  13. 13.

    A. Rev. Biochem. 37, 548–570 (1968).

  14. 14.

    J. molec. Evol. 2, 17–27 (1972).

  15. 15.

    , , & J. biol. Chem. 248, 2876–2879 (1972).

  16. 16.

    , & J. biol. Chem. 246, 305–308 (1971).

  17. 17.

    , & Nature 246, 461–466 (1973).

  18. 18.

    , & J. biol. Chem. 254, 4800–4808 (1979).

  19. 19.

    , , & Cereal Foods Wld 22, 478 (Abstr.) (1977).

  20. 20.

    , , & Cereal Chem. 54, 1070–1083 (1977).

  21. 21.

    , & Cereal Chem. 56, 327–332 (1979).

  22. 22.

    , & Prehistoric Agriculture (ed. Struever, S.) 471 (The American Museum of Natural History, New York, 1971).

  23. 23.

    & Chromosoma (Berl.) 79, 251–270 (1980).

  24. 24.

    & Science 161, 529–540 (1968).

  25. 25.

    Science 191, 528–535 (1976).

  26. 26.

    , & J. molec. Biol. 117, 637–655 (1977).

Download references

Author information

Affiliations

  1. Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA

    • Asim Esen
  2. Northern Regional Research Center, USDA/SEA/AR, Peoria, Illinois 61604, USA

    • Jerold A. Bietz
    • , Jerrold W. Paulis
    •  & Joseph S. Wall

Authors

  1. Search for Asim Esen in:

  2. Search for Jerold A. Bietz in:

  3. Search for Jerrold W. Paulis in:

  4. Search for Joseph S. Wall in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/296678a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.