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Enhanced transformation of human fibroblasts by origin-defective simian virus 40

Nature volume 296, pages 671672 (15 April 1982) | Download Citation

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Abstract

Transformation of semipermissive human fibroblasts (HF) by wild-type simian virus 40 (SV40) or SV40 DNA is relatively inefficient compared with SV40 transformation of non-permissive rodent cells1. Whereas HF transformed with either SV40 or a subgenomic fragment of SV40 (that is incapable of making virions) containing the early region and the origin of DNA replication produce large amounts of free virus DNA2–5, established human cell lines transformed by SV40 harbour defective virus genomes6 that are incapable of initiating virus DNA replication (M. Botchan, personal communication). We have now investigated whether the low efficiency of transformation is directly related to the ability of SV40 DNA to replicate autonomously in semipermissive HF. We have compared the efficiency of transformation of HF by origin-defective SV40 DNA (SV ori) with that of other derivatives containing a functional viral origin of replication, using the calcium phosphate co-precipitation technique7. The transforming potential of SV ori mutants was found to be superior.

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Author information

Affiliations

  1. Department of Biological Sciences, Hunter College, The City University of New York, New York, New York 10021, USA

    • Michael B. Small
    •  & Harvey L. Ozer
  2. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA

    • Yakov Gluzman

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DOI

https://doi.org/10.1038/296671a0

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