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Construction of a functional human suppressor tRNA gene: an approach to gene therapy for β-thalassaemia

Nature volume 296, pages 537540 (08 April 1982) | Download Citation

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Abstract

A human tRNALys gene was converted to an amber suppressor by site-specific mutagenesis of the anticodon. The mutated tRNALys gene directed synthesis of a tRNA that suppressed the UAG amber nonsense mutation in β0 thalassaemia mRNA. Such genes may be used to detect other nonsense mutations in mammalian cells and may provide an approach to gene therapy for β0 thalassaemia due to nonsense mutations.

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Affiliations

  1. Howard Hughes Medical Institute Laboratory, and Departments of Medicine, Biochemistry and Biophysics, University of California, San Francisco, California 94143, USA

    • Gary F. Temple
    • , Andrée M. Dozy
    •  & Yuet Wai Kan
  2. Department of Microbiology, University of Alberta, Edmonton, Alberta, Canada T6G 2E9

    • Kenneth L. Roy
  3. Present address: Bethesda Research Laboratories Gaithersburg, Maryland, USA.

    • Gary F. Temple

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https://doi.org/10.1038/296537a0

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