Abstract
The skeletal framework of the eukaryotic nucleus is a protein matrix1,2 which maintains the physical shape of the nucleus. Attached to the matrix is a fraction of DNA resistant to deoxyribonuclease I (DNase I) digestion. Ribosomal DNA sequences also are enriched on nuclear matrices from rat liver3. The question thus arises whether other repeated DNAs are preferentially attached to the nuclear matrix. This question could be resolved if the repeated DNA was available in sufficient quantity for biochemical studies and differed in its physical or sequence characteristics from the rest of the nuclear DNA. The satellite DNAs of bovine cells meet these criteria. Here we show that nuclear matrices from bovine cells are in fact enriched in satellite DNA.
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Matsumoto, L. Enrichment of satellite DNA on the nuclear matrix of bovine cells. Nature 294, 481–482 (1981). https://doi.org/10.1038/294481a0
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DOI: https://doi.org/10.1038/294481a0
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