Abstract
Experiments using conventional antisera1,2 and monoclonal antibodies3–5 have shown that there are two functionally distinct human T-cell subsets which express unique cell-surface glycoproteins6–9. The inducer population expresses a 62,000-molecular weight (Mr) antigen termed T4 (ref. 6) whereas the cytotoxic/suppressor population lacks T4 but expresses T5 antigen. This antigen is a 76,000-Mr molecule in nonreducing conditions and a 33,000-Mr glycoprotein occasionally appearing as a doublet in reducing conditions. As the functions of subset-specific T-cell surface glycoproteins have not been elucidated, we have now examined the effect on T-cell function of three monoclonal antibodies reactive with the cytotoxic/suppressor subset; anti-T8A antibody markedly inhibited cell-mediated lympholysis (CML) and anti-T8 partially affected CML, whereas anti-T5 had no effect. In contrast, there was no effect on suppressor cell function. Immunoprecipitation studies and competitive binding experiments indicated that anti-T8 and anti-T8A, like anti-T5, defined a 33,000-Mr antigen. Taken together, our results suggest that of a possible family of 33,000-Mr glycoproteins on the human cytotoxic/suppressor T-lymphocyte subset, that which is reactive with anti-T8A has some effect on the cytolytic mechanism.
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Reinherz, E., Hussey, R., Fitzgerald, K. et al. Antibody directed at a surface structure inhibits cytolytic but not suppressor function of human T lymphocytes. Nature 294, 168–170 (1981). https://doi.org/10.1038/294168a0
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DOI: https://doi.org/10.1038/294168a0
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