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Microheterogeneity of protein and sterol content in kidney podocyte membrane

Abstract

Podocytes are highly specialized epithelial cells which form part of the filtration barrier in the kidney glomerulus1. Their cell body sends out long ‘foot processes’ which interdigitate with those of neighbouring podocytes and which rest on the glomerular basal lamina. The maintenance of this characteristic cellular architecture is critical to the normal functioning of the kidney and may be controlled not only by cytoskeletal elements within the cell1,2 but also by some unknown properties of the podocyte plasma membrane. Using freeze-fracture and a cytochemical probe for membrane cholesterol, filipin3,4, we show here that the protein content of and the number of filipin–sterol complexes in the podocyte plasma membrane are markedly reduced at sites of contact with the basal lamina, illustrating a precise zonal membrane differentiation in this region.

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References

  1. 1

    Latta, H. in Handbook of Physiology, Section 8, Renal Physiology (eds Orloff, J. & Berliner, R. W.) 1–29 (American Physical Society, Washington DC, 1973).

    Google Scholar 

  2. 2

    Tyson, G. E. Virchows Arch. path. Anat. Physiol. 25, 105–115 (1977).

    MathSciNet  CAS  Google Scholar 

  3. 3

    Norman, A. W., Demel, R. A., De Kruijff, B. & Van Deenen, L. L. M. J. biol. Chem. 247, 1918–1929 (1972).

    CAS  PubMed  Google Scholar 

  4. 4

    Elias, P. M., Friend, D. S. & Goerke, J. J. Histochem. Cytochem. 27, 1247–1260 (1979).

    CAS  Article  Google Scholar 

  5. 5

    Robinson, J. M. & Karnovsky, M. J. J. Histochem. Cytochem. 28, 161–168 (1980).

    CAS  Article  Google Scholar 

  6. 6

    Brown, D. J. Micros. (Lond.) 121, 283–287 (1981).

    CAS  Article  Google Scholar 

  7. 7

    Moor, H., Mühlethaler, K., Waldner, H. & Frey-Wyssling, A. J. Biophys. biochem. Cytol. 10, 1–13 (1961).

    CAS  Article  Google Scholar 

  8. 8

    Orci, L. & Perrelet, A. Freeze-Etch Histology (Springer, Berlin, 1975).

    Book  Google Scholar 

  9. 9

    Pricam, C., Humbert, F., Amherdt,M. & Orci, L. Lab. Invest. 33, 209–218 (1975).

    CAS  PubMed  Google Scholar 

  10. 10

    Vail, W. J., Papahadjopoulos, D. & Moscarello, M. A. Biochim. biophys. Acta 345, 463–467 (1974).

    CAS  Article  Google Scholar 

  11. 11

    Yu, J. & Branton, D. Proc. natn. Acad. Sci. U.S.A. 73, 3891–3895 (1976).

    ADS  CAS  Article  Google Scholar 

  12. 12

    Robinson, J. M. & Karnovsky, M. J. J. Cell Biol. 87, 562–568 (1980).

    CAS  Article  Google Scholar 

  13. 13

    Ryan, G. B., Leventhal, M. & Karnovsky, M. J. Lab. Invest. 32, 397–403 (1975).

    CAS  PubMed  Google Scholar 

  14. 14

    Caulfield, J. P., Reid, J. J. & Farquhar, M. G. Lab. Invest. 34, 43–59 (1976).

    CAS  PubMed  Google Scholar 

  15. 15

    Montesano, R., Perrelet, A., Vassalli, P. & Orci, L. Proc. natn. Acad. Sci. U.S.A. 76, 6391–6395 (1979).

    ADS  CAS  Article  Google Scholar 

  16. 16

    Branton, D. et al. Science 190, 54–56 (1975).

    ADS  CAS  Article  Google Scholar 

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Orci, L., Singh, A., Amherdt, M. et al. Microheterogeneity of protein and sterol content in kidney podocyte membrane. Nature 293, 646–647 (1981). https://doi.org/10.1038/293646a0

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