Abstract
Harvey murine sarcoma virus (Ha-MuSV), a replication-defective retrovirus, was originally isolated by passage of Moloney murine leukaemia virus (Mo-MuLV) in rats1. The Ha-MuSV genome has recently been molecularly cloned and its DNA physically characterized2–4. Apart from Mo-MuLV sequences at the 5′ and 3′ termini of the viral genome, the other viral sequences are rat derived. One set of rat sequences is homologous to highly reiterated genes encoding ‘rat 30S’ RNA5,6. The biological activity of cloned subgenomic fragments3,4,7 indicated that a second set of rat sequences (src) is essential for transformation and expression of the transforming protein p21, which led us to select a 1.29 kilobase pair (kbp) fragment, totally circumscribing the transforming src gene, for insertion into the late region of an SV40 vector. The data presented here indicate that on transmission to African green monkey kidney (AGMK) cells, this subgenomic fragment is stably expressed as a 930 base pair (bp) mRNA. This apparently unspliced mRNA encodes large quantities of a transforming protein that is indistinguishable from Ha-MuSV p21. A mRNA with a 5′ end similar to that transcribed from the recombinant molecule has also been identified in HaMuSV-transformed mouse cells.
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Gruss, P., Ellis, R., Shih, T. et al. SV40 recombinant molecules express the gene encoding p21 transforming protein of Harvey murine sarcoma virus. Nature 293, 486–488 (1981). https://doi.org/10.1038/293486a0
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DOI: https://doi.org/10.1038/293486a0
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