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Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation

Nature volume 292, pages 311–317 (1981)Cite this article

Abstract

The transcriptionally active ev-3 and inactive ev-1 endogenous retrovirus loci in chick cells differ in that ev-3 is undermethylated, preferentially sensitive to DNase I digestion, and contains nuclease hypersensitive sites in each of its two long terminal repeats. Transient exposure of cells to 5-azacytidine, a cytosine analogue which cannot be methylated at the 5 position, results in the hypomethylation and transcriptional activation of ev-1, as well as the acquisition of at least one nuclease-hypersensitive site within the chromosomal domain of ev-1.

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Authors and Affiliations

  1. Hutchinson Cancer Center, 1124 Columbia Street, Seattle, Washington, 98104, USA

    Mark Groudine, Robert Eisenman & Harold Weintraub

  2. Department of Radiation Oncology, University of Washington Hospital, Seattle, Washington, 98105, USA

    Mark Groudine

Authors
  1. Mark Groudine
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  2. Robert Eisenman
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  3. Harold Weintraub
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Groudine, M., Eisenman, R. & Weintraub, H. Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation. Nature 292, 311–317 (1981). https://doi.org/10.1038/292311a0

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