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Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation

Abstract

The transcriptionally active ev-3 and inactive ev-1 endogenous retrovirus loci in chick cells differ in that ev-3 is undermethylated, preferentially sensitive to DNase I digestion, and contains nuclease hypersensitive sites in each of its two long terminal repeats. Transient exposure of cells to 5-azacytidine, a cytosine analogue which cannot be methylated at the 5 position, results in the hypomethylation and transcriptional activation of ev-1, as well as the acquisition of at least one nuclease-hypersensitive site within the chromosomal domain of ev-1.

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References

  1. Weintraub, H. & Groudine, M. Science 93, 848–858 (1976).

    ADS  Article  Google Scholar 

  2. Garel, A., Zolan, M. & Axel, R. Proc. natn. Acad. Sci. U.S.A. 74, 4867–4871 (1977).

    ADS  CAS  Article  Google Scholar 

  3. Palmiter, R., McKnight, S., Mulvihill, E. & Senear, A. Cold Spring Harb. Symp. quant. Biol. 42, 639–647 (1977).

    Article  Google Scholar 

  4. Stalder, J., Groudine, M., Dodgson, J., Engel, D. & Weintraub, H. Cell 19, 973–980 (1980).

    CAS  Article  Google Scholar 

  5. McGhee, J. & Ginder, G. D. Nature 280, 419–420 (1979).

    ADS  CAS  Article  Google Scholar 

  6. Mandel, J. & Chambon, P. Nucleic Acids Res. 7, 2081–2103 (1979).

    CAS  Article  Google Scholar 

  7. Kuo, T., Mandel, J. & Chambon, P. Nucleic Acids Res. 7, 2105–2113 (1979).

    CAS  Article  Google Scholar 

  8. Bird, A. P., Taggart, M. H. & Smith, B. A. Cell 17, 889–901 (1979).

    CAS  Article  Google Scholar 

  9. Van der Ploag, J. & Flavell, R. Cell 19, 947–958 (1980).

    Article  Google Scholar 

  10. Shen, S. & Maniatis, T. Proc. natn. Acad. Sci. U.S.A. 77, 6634–6638 (1980).

    ADS  CAS  Article  Google Scholar 

  11. Weintraub, H., Larsen, A. & Groudine, M. Cell 24, 333–344 (1981).

    CAS  Article  Google Scholar 

  12. Naveh, T. & Cedar, H. Proc. natn. Acad. Sci. U.S.A. (in the press).

  13. Wu, C., Bingham, P. M., Livak, K., Holmgren, R. & Elgin, S. C. R. Cell 16, 797–806 (1979).

    CAS  Article  Google Scholar 

  14. Wu, C. Nature 284, 856–860 (1980).

    Google Scholar 

  15. Stalder, J. et al. Cell 20, 451–460 (1980).

    CAS  Article  Google Scholar 

  16. Groudine, M. & Weintraub, H. Cell 24, 393–401 (1981).

    CAS  Article  Google Scholar 

  17. Astrin, S. Proc. natn. Acad. Sci. U.S.A. 75, 5941–5945 (1978).

    ADS  CAS  Article  Google Scholar 

  18. Skalka, A., DeBona, P., Hishinuma, F. & McClements, W. Cold Spring Harb. Symp. quant. Biol. 44, 1097–1104 (1980).

    CAS  Article  Google Scholar 

  19. Hughes, S., Toyoshima, K., Bishop, J. & Varmous, H. Virology 108, 189–207 (1981).

    CAS  Article  Google Scholar 

  20. Hayward, W., Brauerman, S. & Astrin, S. Cold Spring Harb. Symp. quant. Biol. 44, 1111–1121 (1980).

    CAS  Article  Google Scholar 

  21. Astrin, S. et al. Cold Spring Harb. Symp. quant. Biol. 44, 1105–1109 (1980).

    CAS  Article  Google Scholar 

  22. Wang, S., Hayward, W. & Hanafusa, H. J. Virol. 24, 64–73 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  23. Eisenman, R., Shaikh, R. & Mason, W. S. Cell 14, 889–904 (1978).

    Article  Google Scholar 

  24. Eisenman, R., Mason, W. & Linial, M. J. Virol. 36, 62–78 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  25. Jones, P. & Taylor, S. Cell 20, 85–93 (1980).

    CAS  Article  Google Scholar 

  26. Groudine, M., Das, S., Neiman, P. & Weintraub, H. Cell 14, 865–878 (1978).

    CAS  Article  Google Scholar 

  27. Ju, G. & Shalka, A. Cell 22, 379–386 (1980).

    CAS  Article  Google Scholar 

  28. Yamamoto, T., de Crombrugghe, B. & Paston, I. Cell 22, 787–797 (1980).

    CAS  Article  Google Scholar 

  29. Waalwyck, C. & Flavell, R. A. Nucleic Acids Res. 5, 4631–4641 (1978).

    Article  Google Scholar 

  30. Humphries, E., Glover, C., Weiss, R. & Arrand, J. Cell 18, 803–815 (1979).

    CAS  Article  Google Scholar 

  31. Guntaka, R., Rao, P., Mitsialis, S. & Katz, R. J. Virol. 34, 569–572 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  32. Cohen, J. C. Cell 19, 653–662 (1980).

    CAS  Article  Google Scholar 

  33. Akiyama, Y. & Kato, S. Biken J. 17, 105–116 (1974).

    CAS  PubMed  Google Scholar 

  34. Nazerian, K. & Lee, L. J. gen. Virol. 25, 317–321 (1974).

    CAS  Article  Google Scholar 

  35. Thomas, P. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).

    ADS  CAS  Article  Google Scholar 

  36. Nazerian, K., Neiman, P., Okazaki, A., Smith, E. & Crittenden, L. Avian Dis. 22, 732–741 (1978).

    CAS  Article  Google Scholar 

  37. Varshavsky, A., Sundin, O. & Bohn, M. Cell 16, 453–466 (1979).

    CAS  Article  Google Scholar 

  38. Weisbrod, S., Groudine, M. & Weintraub, H. Cell 19, 289–301 (1980).

    CAS  Article  Google Scholar 

  39. Weintraub, H. Nucleic Acids Res. 8, 4745–4754 (1980).

    CAS  Article  Google Scholar 

  40. Benoist, C. & Chambon, P. Nature 290, 304–309 (1981).

    ADS  CAS  Article  Google Scholar 

  41. Sundin, O. & Varshavsky, A. J. molec. Biol. 132, 535–546 (1979).

    CAS  Article  Google Scholar 

  42. Varshavsky, A., Sundin, O. & Bahn, M. Nucleic Acids Res. 5, 3469–3477 (1978).

    CAS  Article  Google Scholar 

  43. Blair, D., Mclements, W., Oskarsson, M., Fischarger, P. & Vande Wande, G. Proc. natn. Acad. Sci. U.S.A. 77, 3504–3508 (1980).

    ADS  CAS  Article  Google Scholar 

  44. Neel, B., Hayward, W., Robinson, H., Fang, J. & Astrin, S. Cell 23, 323–334 (1981).

    CAS  Article  Google Scholar 

  45. Schmitz, A. & Galas, D. Nucleic Acids Res. 6, 111–137 (1979).

    CAS  Article  Google Scholar 

  46. Weiss, R., Friis, R., Katz, E. & Vogt, P. Virology 46, 920–938 (1971).

    CAS  Article  Google Scholar 

  47. Robinson, H., Swanson, C., Hruska, J. & Crittendon, L. Virology 69, 63–74 (1976).

    CAS  Article  Google Scholar 

  48. Christman, J., Price, P., Pedriman, L. & Acs, G. Eur. J. Biochem. 81, 53–61 (1977).

    CAS  Article  Google Scholar 

  49. Chiang, P., Science 211, 1164–1166 (1981).

    ADS  CAS  Article  Google Scholar 

  50. Lowy, D. Proc. natn. Acad. Sci. U.S.A. 75, 5539–5543 (1978).

    ADS  CAS  Article  Google Scholar 

  51. Razin, A. & Riggs, A. Science 210, 604–610 (1980).

    ADS  CAS  Article  Google Scholar 

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Groudine, M., Eisenman, R. & Weintraub, H. Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation. Nature 292, 311–317 (1981). https://doi.org/10.1038/292311a0

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