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Establishment in culture of pluripotential cells from mouse embryos


Pluripotential cells are present in a mouse embryo until at least an early post-implantation stage, as shown by their ability to take part hi the formation of chimaeric animals1 and to form teratocarcinomas2. Until now it has not been possible to establish progressively growing cultures of these cells in vitro, and cell lines have only been obtained after teratocarcinoma formation in vivo. We report here the establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts. These cells are able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo. They have a normal karyotype.

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  1. Gardner, R. L. & Papaioannou, V. E. in The Early Development of Mammals (eds Balls, M. & Wild, A. E.) 107–132 (Cambridge University Press, 1975).

    Google Scholar 

  2. Stevens, L. C. Devl Biol 21, 364–382 (1970).

    CAS  Article  Google Scholar 

  3. Cole, R. J. & Paul, J. in Preimplantation Stages of Pregnancy (eds Wolstenholme, G. E. W. & O'Connor, M.) 82–122 (Churchill, London, 1965).

    Google Scholar 

  4. Sherman, M. I. Cell 5, 343–349 (1975).

    CAS  Article  Google Scholar 

  5. Atienza-Samols, S. B. & Sherman, M. I. Devl Biol 66, 220–231 (1978).

    CAS  Article  Google Scholar 

  6. Solter, D. & Knowles, B. Proc. natn. Acad. Sci. U.S.A. 72, 5099–5102 (1975).

    ADS  CAS  Article  Google Scholar 

  7. Evans, M. J. J. Reprod. Pert. 62, 625–631 (1981).

    CAS  Article  Google Scholar 

  8. Evans, M. J., Lovell-Badge, R. H., Stern, P. L. & Stinnakre, M.-G. INSERM Symp. 10, 115–129(1979).

    Google Scholar 

  9. McLaren, A. J. Endocr. 50, 515–526 (1971).

    CAS  Article  Google Scholar 

  10. Kaufman, M. H. in Progress in Anatomy Vol. 1 (eds Harrison, R. J. & Holmes, R. L.) 1–34 (Cambridge University Press, 1981).

    Google Scholar 

  11. Stern, P. L. et al. Cell 14, 775–783 (1978).

    CAS  Article  Google Scholar 

  12. Feizi, T. Blood Transfusion Immunohaemat. 23, 563–577 (1980).

    CAS  Google Scholar 

  13. Kapadia, A., Feizi, T. & Evans, M. J. Expl Cell Res. 131, 185–195 (1980).

    Article  Google Scholar 

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Evans, M., Kaufman, M. Establishment in culture of pluripotential cells from mouse embryos. Nature 292, 154–156 (1981).

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