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SV40 T antigen binds specifically to a cellular 53 K protein in vitro

Nature volume 292, pages 63–65 (1981)Cite this article

Abstract

Simian virus 40 (SV40) large T antigen is a multifunctional protein that is essential for virus replication and the establishment and maintenence of cell transformation1. The molecular basis of these functions probably involves specific interactions of T antigen with DNA2–4 and cellular proteins. An example of such an interaction has been described recently; a significant fraction of the T antigen extracted from cell lysates is tightly bound to a cellular phosphoprotein of molecular weight 53,000 (53K)5–9. Here we demonstrate in vitro the direct interaction of highly purified T antigen (D2 T antigen or SV80 T antigen) with 53K in lysates from a variety of mouse cell lines. All the 53K detected in these lysates was able to bind to added T antigen, and it was the only cellular protein with which T antigen formed a stable complex. We conclude that 53K binds directly to a specific site on T antigen and that binding occurs without previous modification and without involvement of other virus-encoded or induced proteins.

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Authors and Affiliations

  1. Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2A 3PX, UK

    Frank McCormick & Ed Harlow

  2. Department of Biochemistry, University of California, Berkeley, California, 94720, USA

    Robin Clark & Robert Tjian

Authors
  1. Frank McCormick
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  2. Robin Clark
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  3. Ed Harlow
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  4. Robert Tjian
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McCormick, F., Clark, R., Harlow, E. et al. SV40 T antigen binds specifically to a cellular 53 K protein in vitro. Nature 292, 63–65 (1981). https://doi.org/10.1038/292063a0

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