Primary structure, gene organization and polypeptide expression of poliovirus RNA

Abstract

The primary structure of the poliovirus genome has been determined. The RNA molecule is 7,433 nucleotides long, polyadenylated at the 3′ terminus, and covalently linked to a small protein (VPg) at the 5′ terminus. An open reading frame of 2,207 consecutive triplets spans over 89% of the nucleotide sequence and codes for the viral polyprotein NCVPOO. Twelve viral polypeptides have been mapped by amino acid sequence analysis and were found to be proteolytic cleavage products of the polyprotein, cleavages occurring predominantly at Gln-Gly pairs.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Landsteiner, K. & Popper, E. Wien. Klin. Wochenschr. 21, 1830 (1908).

  2. 2

    Salk, J. & Salk, D. Science 195, 834–847 (1977).

  3. 3

    Sabin, A. B. & Boulger, C. R. J. biol. Standard 1, 115–118 (1973).

  4. 4

    Lee, Y. F., Nomoto, A. & Wimmer, E. Prog. Nucleic Acid Res. molec. Biol. 19, 89–95 (1976).

  5. 5

    Lee, Y. F., Nomoto, A., Detjen, B. M. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 74, 59–63 (1977).

  6. 6

    Nomoto, A., Detjen, B., Pozzatti, R. & Wimmer, E. Nature 268, 208–213 (1977).

  7. 7

    Semler, B. L. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).

  8. 8

    Larsen, G. R., Semler, B. L. & Wimmer, E. J. Virol. 37, 328–335 (1981).

  9. 9

    Pettersson, R. F., Flanegan, J. B., Rose, J. K. & Baltimore, D. Nature 268, 270–272 (1977).

  10. 10

    Nomoto, A., Kitamura, N., Golini, F. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 74, 5345–5349 (1977).

  11. 11

    Jacobson, M. F. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 61, 77–84 (1968).

  12. 12

    Summers, D. F. & Maizel, J. V. Proc. natn. Acad. Sci. U.S.A. 59, 966–971 (1968).

  13. 13

    Holland, J. J. & Kiehn, E. D. Proc. natn. Acad. Sci. U.S.A. 60, 1015–1022 (1968).

  14. 14

    Rueckert, R. R. Compreh. Virol. 6, 131–213 (1976).

  15. 15

    Cooper, P. D. Compreh. Virol. 9, 133–207 (1977).

  16. 16

    Kates, J. Cold Spring Harb. Symp. quant. Biol. 35, 743–752 (1970).

  17. 17

    Armstrong, J. A., Edmonds, M., Nakazato, H., Philips, B. A. & Vaughan, M. H. Science 176, 526–528 (1972).

  18. 18

    Yogo, Y. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 69, 1877–1882 (1972).

  19. 19

    Kacian, D. L. & Myers, J. C. Proc. natn. Acad. Sci. U.S.A. 73, 2191–2195 (1976).

  20. 20

    Kitamura, N. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 77, 3196–3200 (1980).

  21. 21

    Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

  22. 22

    Sanger, F. & Coulson, A. R. FEBS Lett. 87, 107–116 (1978).

  23. 23

    Nomoto, A., Jacobson, A., Lee, Y. F., Dunn, J. J. & Wimmer, E. J. molec. Biol. 128, 179–196 (1979).

  24. 24

    Nomoto, A. et al. Virology (in the press).

  25. 25

    Kramer, F. R. & Mills, D. R. Proc. natn. Acad. Sci. U.S.A. 75, 5334–5338 (1978).

  26. 26

    Flanegan, J. B., Pettersson, R. F., Ambros, V., Hewlett, J. J. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 74, 961–965 (1977).

  27. 27

    Hewlett, M. J. & Florkiewicz, R. Z. Proc. natn. Acad. Sci. U.S.A. 77, 303–307 (1980).

  28. 28

    Nomoto, A. & Imura, N. Nucleic Acids Res. 7, 1233–1246 (1979).

  29. 29

    Nomoto, A. et al. J. molec. Biol. 128, 165–177 (1979).

  30. 30

    Harris, T. J. R., Dunn, J. J. & Wimmer, E. Nucleic Acids Res. 5, 4039–4054 (1978).

  31. 31

    Nomoto, A., Lee, Y. F. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 73, 375–380 (1976).

  32. 32

    Rothberg, P. G., Harris, T. J. R., Nomoto, A. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 75, 4868–4872 (1978).

  33. 33

    Ambros, V. & Baltimore, D. J. biol. Chem. 253, 5263–5266 (1978).

  34. 34

    Wu, M., Davidson, N. & Wimmer, E. Nucleic Acids Res. 5, 4711–4723 (1978).

  35. 35

    Golini, F., Nomoto, A. & Wimmer, E. Virology 89, 112–118 (1978).

  36. 36

    Kitamura, N. et al. Cell 21, 295–302 (1980).

  37. 37

    Pallansch, M. A. et al. J. Virol. 35, 414–419 (1980).

  38. 38

    Holzer, H. & Wohlhueter, R. Adv. Enzyme Reg. 10, 121–132 (1972).

  39. 39

    Yogo, Y. & Wimmer, E. J. molec. Biol. 92, 467–477 (1975).

  40. 40

    Pettersson, R. F., Ambros, V. & Baltimore, D. J. Virol. 27, 357–365 (1978).

  41. 41

    Wimmer, E. in The Molecular Biology of Picornaviruses (ed. Pérez-Bercoff, R.) 175–190 (Plenum, New York, (1979).

  42. 42

    Kitamura, N., Adler, C. & Wimmer, E. Ann. N.Y. Academy Sci. 354, 183–201 (1980).

  43. 43

    Ehrenfeld, E. in The Molecular Biology of Picornaviruses (ed. Pérez-Bercoff, R.) 223–238 (Plenum, New York, (1979).

  44. 44

    Steitz, J. A. in RNA Phages (ed. Zinder, N. D.) 319–352 (Cold Spring Harbor Laboratory, New York, (1975).

  45. 45

    Golini, F., Semler, B. L., Dorner, A. J. & Wimmer, E. Nature 287, 600–603 (1980).

  46. 46

    Shine, J. & Dalgarno, L. Proc. natn. Acad. Sci. U.S.A. 71, 1342–1346 (1974).

  47. 47

    Kozak, M. in Protein Biosynthesis in Eukaryotes (Plenum, New York, in the press).

  48. 48

    Palmenberg, A. C., Pallansch, M. A. & Rueckert, R. R. J. Virol. 32, 770–778 (1979).

  49. 49

    Svitkin, Yu. V., Gorbalenya, A. E., Kazachkov, Yu. A. & Agol, V. I. FEBS Lett. 108, 6–9 (1979).

  50. 50

    Lundquist, R. E., Ehrenfeld, E. & Maizel, J. V. Jr Proc. natn. Acad. Sci. U.S.A. 71, 4773–4777 (1974).

  51. 51

    Flanegan, J. B. & Baltimore, D. J. Virol. 29, 352–360 (1979).

  52. 52

    Wouters, M. & Vandekerckhove, J. J. gen. Virol. 33, 529–533 (1976).

  53. 53

    Wetz, K. & Habermehl, K. O. J. gen. Virol. 44, 525–534 (1979).

  54. 54

    Andersen, C. W. & Lewis, J. B. Virology 104, 27–41 (1980).

  55. 55

    Sanger, D.V., Black, D.N., Rowlands, D.J., Harris, T.J.R. & Brown, F. J. Virol. 33, 59–68 (1980).

  56. 56

    Vrijsen, R., Boéye, A. & Strosberg, A. D. Biochem. biophys. Res. Commun. 85, 1596–1601 (1978).

  57. 57

    Heukeshoven, J. & Dernick, R. Z. Naturforschung 36, 164–172 (1981).

  58. 58

    Rueckert, R. R., Matthews, T. J., Kew, O. M., Pallansch, M., McLean, C. & Omilianowski, D. in The Molecular Biology of Picornaviruses (ed. Pérez-Bercoff, R.) 113–125 (Plenum, New York, (1979).

  59. 59

    Fitzgerald, M. & Shenk, T. Cell 24, 251–260 (1981).

  60. 60

    Yogo, Y., Teng, M. H. & Wimmer, E. Biochem. biophys. Res. Commun. 61, 1101–1109 (1974).

  61. 61

    Spector, D. H. & Baltimore, D. Virology 67, 498–505 (1975).

  62. 62

    Dorsch-Häsler, K., Yogo, Y. & Wimmer, E. J. Virol. 16, 1512–1527 (1975).

  63. 63

    Ahlquist, P. & Kaesberg, P. Nucleic Acids Res. 7, 1195–1204 (1979).

  64. 64

    Spector, D. H. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 71, 2983–2987 (1974).

  65. 65

    Dasgupta, A., Zabel, P. & Baltimore, D. Cell 19, 423–429 (1980).

  66. 66

    Porter, A. G. et al. Nature 276, 298–301 (1978).

  67. 67

    Korant, B. D., Chow, N., Lively, M. & Powers, J. Ann. N.Y. Acad. Sci. 343, 304–315 (1980).

  68. 68

    Pelham, H. R. B. Eur. J. Biochem. 85, 457–462 (1978).

  69. 69

    Rueckert, R. R., Palmenberg, A. C. & Pallansch, M. A. Eur. J. cell. Biol. 22, 263–275 (1980).

  70. 70

    Etchison, D. & Ehrenfeld, E. Virology 107, 135–143 (1980).

  71. 71

    Anfinsen, C. B. & Scheraga, H. H. Adv. Protein Chem. 25, 205–300 (1975).

  72. 72

    Korant, B., Chow, N., Lively, M. & Powers, J. Proc. natn. Acad. Sci. U.S.A. 76, 2992–2995 (1979).

  73. 73

    Scraba, D. G. in The Molecular Biology of Picornaviruses (ed. Pérez-Bercoff, R.) 1–23 (Plenum, New York, (1979).

  74. 74

    Burrel, C. J. & Cooper, P. D. J. gen. Virol. 21, 443–451 (1973).

  75. 75

    Van der Werf, S. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).

  76. 76

    Boothroyd, J. C. et al. Nature 290, 800–802 (1981).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kitamura, N., Semler, B., Rothberg, P. et al. Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature 291, 547–553 (1981). https://doi.org/10.1038/291547a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.