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Primary structure, gene organization and polypeptide expression of poliovirus RNA

Nature volume 291, pages 547–553 (1981)Cite this article

Abstract

The primary structure of the poliovirus genome has been determined. The RNA molecule is 7,433 nucleotides long, polyadenylated at the 3′ terminus, and covalently linked to a small protein (VPg) at the 5′ terminus. An open reading frame of 2,207 consecutive triplets spans over 89% of the nucleotide sequence and codes for the viral polyprotein NCVPOO. Twelve viral polypeptides have been mapped by amino acid sequence analysis and were found to be proteolytic cleavage products of the polyprotein, cleavages occurring predominantly at Gln-Gly pairs.

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Author notes

  1. Naomi Kitamura

    Present address: Department of Viral Oncology, Cancer Institute, 1–37–1 Kami-Ikebukuru, Toshima-ku, Tokyo, 170, Japan

  2. James J. Lee

    Present address: Department of Biology, California Institute of Technology, Pasadena, California, 91125, USA

  3. Sylvie van der Werf

    Present address: Unité de Virologie Moléculaire et Unité de Génie Génétique, Institut Pasteur, Paris, France

Authors and Affiliations

  1. Department of Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, New York, 11794, USA

    Naomi Kitamura, Bert L. Semler, Paul G. Rothberg, Glenn R. Larsen, Cheryl J. Adler, Andrew J. Dorner, Emilio A. Emini, Ronnie Hanecak, James J. Lee, Sylvie van der Werf & Eckard Wimmer

  2. Department of Biology, Brookhaven National Laboratory, Upton, New York, 11973, USA

    Carl W. Anderson

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Kitamura, N., Semler, B., Rothberg, P. et al. Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature 291, 547–553 (1981). https://doi.org/10.1038/291547a0

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