Abstract
The adhesion of cells to the connective tissue matrix is commonly thought to be governed by fibronectin, a pericellular glycoprotein with binding sites for cell surfaces, collagen and glycosaminoglycans. Here we report evidence that Chinese hamster ovary (CHO) cells possess an alternative mechanism for adhesion which is independent of fibronectin. Cells of a variant CHO clone called ADVF11 are defective in their ability to adhere to fibronectin-coated substrata, but can adhere to a substratum coated with SAM (substrate-attached material), a pericellular material produced by fibroblasts. The adhesion of wild-type CHO cells to fibronectin-coated substrata and adhesion of ADVF11 cells to SAM-coated substrata are differentially sensitive to proteolytic treatment. This suggests that there are two distinct adhesion mechanisms for CHO cells, only one of which is dependent on fibronectin.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Grinell, F. Int. Rev. Cytol. 53, 65–144 (1978).
Vaheri, A. & Mosher, D. F. Biochim. biophys. Acta 51, 1–25 (1978).
Yamada, K. M. & Olden, K. Nature 275, 179–184 (1978).
Engvall, E., Ruoslahti, E. & Miller, E. J. J. exp. Med. 147, 1584–1595 (1978).
Kleinman, H. K. et al. J. biol. Chem. 253, 5642–5646 (1978).
Hann L-H. E. & Yamada, K. M. Cell 18, 1043–1051 (1979).
Ruoslahti, E., Hayman, E. G., Kuusela, P., Shively, J. E. & Engvall, E. J. biol. Chem. 254, 6054–6059 (1979).
Balian, G., Click, E. M., Crouch, E., Davidson, J. M. & Bornstein, P. J. biol. Chem. 254, 1429–1482 (1979).
Gold, L. I., Garcia-Pardo, A., Frangione, B., Franklin, E.C. & Pearlstein, E. Proc. natn. Acad. Sci. U. S. A. 76, 4803–4807 (1979).
Stathakis, N. E. & Mosesson, M. W. J. clin. Invest. 60, 855–865 (1977).
Perkins, M. E., Ji, T. H. & Hynes, R. O. Cell 16, 941–952 (1979).
Ruoslahti, E. & Vaheri, A. J. exp. Med. 141, 497–501 (1975).
Kleinman, H. K., Martin, G. R. & Fishman, P. H. Proc. natn. Acad. Sci. U.S.A. 76, 3367–3371 (1979).
Yamada, K. M. & Kennedy, D. W. J. Cell Biol. 80, 492–493 (1979).
Yamada, K. M., Yamada, S. S. & Pastan, I. Proc. natn. Acad. Sci. U.S.A. 72, 1217–1221 (1976).
Harper, P. A. & Juliano, R. L. J. Cell Biol. 87, 755–763 (1980).
Culp, L. A. J. Cell Biol. 63, 71–83 (1974).
Culp, L. A. in Curr. Topics Membranes Transport 11, 327–396 (1978).
Rollins, B. J. & Culp, L. A. Biochemistry 18, 141–148 (1979).
Cathcart, M. K. & Culp, L. A. Biochemistry 18, 1167–1176 (1979).
Revel, J. P., Hoch, P., Ho, P. & Ho, D. Expl Cell Res. 84, 207–218 (1974).
Culp, L. A., Murray, B. A. & Rollins, B. J. J. supramolec. Struct. 11, 391–400 (1979).
Juliano, R. L. & Gagalang, E. J. Cell Physiol. 92, 209–220 (1977).
Engvall, E. & Ruoslahti, E. Int. J. Cancer 20, 1–5 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Harper, P., Juliano, R. Two distinct mechanisms of fibroblast adhesion. Nature 290, 136–138 (1981). https://doi.org/10.1038/290136a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/290136a0
This article is cited by
-
Cyclic adenosine monophosphate modulates cell morphology and behavior of a cultured renal epithelial
Pediatric Nephrology (1990)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.