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Isolation of a gene from Drosophila by complementation in yeast

Abstract

Transformation of mutant yeast cells by cloned genomic DNA from a higher eukaryote has made it possible to isolate a Drosophila DNA sequence that complements a yeast adenine-8 mutation. A 0.8-kilobase poly(A)-containing RNA is transcribed from the cloned Drosophila segment in transformed yeast cells and can account for functional expression of the gene.

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References

  1. Hinnen, A., Farabaugh, P., Ilgen, C., Fink, G. R. & Friesen, J. ICN-UCLA Symp. molec. cell. Biol. 14, 43–50 (1979).

    CAS  Google Scholar 

  2. Broach, J. R., Strathern, J. N. & Hicks, J. B. Gene 8, 121–133 (1979).

    Article  CAS  Google Scholar 

  3. Williamson, V. M., Bennetsen, J., Young, E. T., Nasmyth, K. & Hall, B. D. Nature 283, 214–216 (1980).

    Article  ADS  CAS  Google Scholar 

  4. Nasmyth, K. A. & Reed, S. I. Proc. natn. Acad. Sci. U.S.A. 77, 2119–2123 (1980).

    Article  ADS  CAS  Google Scholar 

  5. Elgin, S. C. R. & Miller, D. W. in The Genetics and Biology of Drosophila Vol. 2a (eds Ashburner, M. E. & Wright, T. R. F.) 145–150 (Academic, New York, 1978).

    Google Scholar 

  6. Bolivar, F. et al. Gene 2, 95–113 (1977).

    Article  CAS  Google Scholar 

  7. Beggs, J. D. Nature 275, 104–108 (1978).

    Article  ADS  CAS  Google Scholar 

  8. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  9. Helling, R. B., Goodman, H. M. & Boyer, H. W. J. Virol. 14, 1235–1244 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Wahl, G. M., Stern, M. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 76, 3683–3687 (1979).

    Article  ADS  CAS  Google Scholar 

  11. Wensink, P. C., Finnegan, D. J., Donelson, J. E. & Hogness, D. S. Cell 3, 315–325 (1974).

    Article  CAS  Google Scholar 

  12. Henikoff, S. & Meselson, M. Cell 12, 441–451 (1977).

    Article  CAS  Google Scholar 

  13. Lindsley, D. L. & Grell, E. H. Genetic Variations of Drosophila melanogaster Publ. No. 627 (Carnegie Institution of Washington, 1968).

    Google Scholar 

  14. Woods, R. & Jackson, I. Biochem. biophys. Res. Commun. 53, 787–793 (1973).

    Article  CAS  Google Scholar 

  15. Thomas, P. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).

    Article  ADS  CAS  Google Scholar 

  16. Schultz, L. D. Biochemistry 17, 750–758 (1978).

    Article  CAS  Google Scholar 

  17. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  18. Berk, A. J. & Sharp, P. A. Cell 12, 721–732.

  19. Holmgren, R., Livak, K., Morimoto, R., Freund, R. & Meselson, M. Cell 18, 1359–1370 (1979).

    Article  CAS  Google Scholar 

  20. Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

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Henikoff, S., Tatchell, K., Hall, B. et al. Isolation of a gene from Drosophila by complementation in yeast. Nature 289, 33–37 (1981). https://doi.org/10.1038/289033a0

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